Amplified electrochemiluminescence of Ru(dcbpy)₃²⁺ via coreactant active sites on nitrogen-doped graphene quantum...

Searching for new alternative to tripropylamine (TPrA) with low toxicity and high chemical stability for the tris(4,4'-dicarboxylic acid-2,2'-bipyridyl)ruthenium (II) (Ru(dcbpy)₃²⁺) based coreactant electrochemiluminescence (ECL) system is essential for widespread analytical applications. Here, nitrogen-doped graphene quantum dots (NGQDs) have been discovered to significantly amplify the ECL emission and increase the ECL efficiency of Ru(dcbpy)₃²⁺ for the first time. However, the mechanism by which NGQDs act as coreactants is not well comprehended. Therefore, various optical and electrochemical technologies were employed to investigate the ECL mechanism. It is proposed that the amino and carboxyl groups on the surface of NGQDs play crucial roles as the coreactant active sites, catalyzing the oxidation of Ru(dcbpy)₃²⁺. Based on this foundation, an "on-off-on" ECL aptasensor for the quantification of acetamiprid was developed, exhibiting a broad linear range and a detection limit of 0.056 pM. Satisfactory recoveries, ranging from 98.0 % to 101.6 %, were achieved in pakchoi samples. Consequently, NGQDs could serve as coreactants for Ru(dcbpy)₃²⁺, offering new opportunities for constructing a variety of sensors with extensive analytical applications in the ECL field.