Assessment of the pathogenicity of Y. enterocolitica B1A isolates from San Luis, Argentina

Yersinia enterocolitica, a bacterial enteropathogen that produces a variety of clinical manifestations in humans, includes six biotypes (B), called 1A, 1B, 2, 3, 4 and 5 and about 70 serotypes. The biotypes exhibit diverse pathogenic potential; while 1B and 2-5 may show ability to produce clinical symptoms due to the presence of chromosomal and plasmid (pYV) virulence genes, B1A is supposed a non-pathogenic biotype since it lacks pYV plasmid. Therefore, although B1A strains cause diarrhea in humans, their pathogenic potential has not yet been extensively studied. The objective of this study was to assess virulence genetic markers of local Y. enterocolitica B1A strains and determine clonal relationships between isolates. To this, Y. enterocolitica chromosomal virulence markers were evaluated by PCR, Yst enterotoxin activity of culture filtrates in the intestine of suckling mice was tested and PFGE was applied in 24 Y. enterocolitica B1A strains obtained from human feces, foods, animals and environmental samples of our region (isolated among 2000-2014). The detection frequency of virulence chromosomal markers was as follows: fepA [95.8% (23/24)], ystB [91.7% (22/24)], hreP [87.5 % (21/24)], tccC [12.5% (3/24)] and myfA [4.2% (1/24)]. Presence of ystB gene was strongly associated to the Yst activity in suckling mice. By PFGE, B1A strains were divided into 10 genomic patterns (GP). Interestingly, human strains showed 88% similarity when compared to strains of the same serotype from other sources. Our results support the pathogenicity of Y. enterocolitica B1A strains and highlight the valuable impact of the Y. enterocolitica monitoring to prevent and control the spreading of this pathogen in our region.