Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

B3GALT4-dependent gangliosides are required for normal late spermatogenesis and male fertility.

The Journal of reproduction and development·2026
Same author

Efficacy and Safety of Balloon Endoscopy-Assisted Peroral Cholangiopancreatoscopy (POCPS) Using a Novel 9Fr Cholangioscope in Patients With Surgically Altered Anatomy: A Dual-Center Retrospective Study (With Video).

Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society·2026
Same author

Overconfluent culture promotes cell cycle synchronization in mouse embryonic stem cells for efficient cloned embryo production.

The Journal of reproduction and development·2026
Same author

A CRISPR knockout mouse library for functional genomics in influenza research.

Cell·2026
Same author

Functional analysis of genes enriched in male reproductive organs: validation via fertility assessment of 14 knockout mouse lines.

Frontiers in cell and developmental biology·2026
Same author

The SPARK complex forms the molecular basis of vertebrate fertilization.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 2, 2025

Intracranial Injection of Adeno-associated Viral Vectors
08:47

Intracranial Injection of Adeno-associated Viral Vectors

Published on: November 17, 2010

47.4K

Trophectoderm-specific gene manipulation using adeno-associated viral vectors.

Tatsuya Nakagawa1,2, Chihiro Emori1, Masahito Ikawa1,2,3,4,5

  • 1Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.

Experimental Animals
|January 13, 2025
PubMed
Summary

Researchers developed a new method for gene expression in the placenta using adeno-associated virus serotype 1 (AAV1). This technique enables trophoblast-specific gene modification without zona pellucida removal, advancing implantation and placenta research.

Keywords:
adeno-associated virusimplantationplacentatrophectoderm

More Related Videos

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research
06:41

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research

Published on: October 20, 2023

2.8K
Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

28.4K

Related Experiment Videos

Last Updated: Jun 2, 2025

Intracranial Injection of Adeno-associated Viral Vectors
08:47

Intracranial Injection of Adeno-associated Viral Vectors

Published on: November 17, 2010

47.4K
Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research
06:41

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research

Published on: October 20, 2023

2.8K
Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

28.4K

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Trophoblast (TE) and placenta are crucial for mammalian fetal development and implantation.
  • Current genetic modification methods in mice affect both fetus and placenta, limiting specific TE/placenta studies.
  • Previous lentiviral vector methods required zona pellucida removal, posing a technical challenge.

Purpose of the Study:

  • To develop a novel method for TE/placenta-specific gene transduction.
  • To overcome the limitation of zona pellucida removal in previous gene delivery techniques.
  • To enable efficient and uniform genetic modification of TE cells for placental research.

Main Methods:

  • Screening of various adeno-associated viral (AAV) vectors for TE/placenta-specific gene transduction.
  • Utilizing AAV1's ability to penetrate the zona pellucida (ZP).
  • Transducing blastocysts and analyzing gene expression and placental contribution.

Main Results:

  • Adeno-associated virus serotype 1 (AAV1) successfully transduced TE cells without requiring ZP removal.
  • AAV1 facilitated trophoblast-specific gene expression.
  • Genetically modified TE cells by AAV1-Cre contributed uniformly to the placenta.

Conclusions:

  • A new, efficient method for TE/placenta-specific gene transduction using AAV1 has been established.
  • This technology eliminates the need for zona pellucida removal, simplifying genetic manipulation.
  • The findings advance research in implantation, placental development, and assisted reproductive technologies.