Related Experiment Video
Updated: Jun 2, 2025

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
Published on: January 26, 2015
Dissecting SNARE-Mediated Exocytosis in RBL-2H3 Mast Cells
1Gene Therapy Center, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Pratikshya_Adhikari@med.unc.edu.
Abstract:
SNARE-dependent mast cell (MC) exocytosis causes the release of a wide variety of mediators with important physiological/pathological consequences. Unlike synaptic transmission in the brain, which relies primarily on one set of exocytic SNAREs (i.e., Syntaxin1, SNAP-25, and VAMP2), MCs produce a multitude of exocytic SNAREs that can form a minimum of 8 distinct sets of fusogenic trans-SNARE complexes. Here we describe the genetic approaches we have developed to dissect the specific roles of these SNAREs in RBL-2H3 cells, a widely utilized model for studying MC signaling and exocytosis.
Related Concept Videos
SNAREs and Membrane Fusion
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
Fusion of Secretory Vesicles with the Plasma Membrane
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
Pinching-off of Coated Vesicles
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...

