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Integrated bioinformatics and experimental analysis of CHAF1B as a novel biomarker and immunotherapy target in LUAD.

Wei Du1, Xiao-Wei Wu2, Qing-Feng Li1

  • 1The School Public Health, Fujian Medical University, Fuzhou, 350122, Fujian, China.

Discover Oncology
|January 13, 2025
PubMed
Summary

Chromosomal assembly factor 1B (CHAF1B) is a potential biomarker for lung adenocarcinoma (LUAD). Suppressing CHAF1B reduced LUAD cell migration, invasion, proliferation, and glycolysis, suggesting it as a therapeutic target.

Keywords:
BiomarkersCHAF1BDrug sensitivityImmunotherapy targetsLung adenocarcinomaPrognosis

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung adenocarcinoma (LUAD) has a high incidence, but prognosis and treatment efficacy are unsatisfactory.
  • Identifying novel biomarkers and therapeutic targets for LUAD is crucial for improving patient outcomes.
  • Chromosomal assembly factor 1B (CHAF1B) is linked to tumor incidence and proliferation, but its role in LUAD is unclear.

Purpose of the Study:

  • To investigate the expression, biological role, and molecular mechanism of CHAF1B in LUAD.
  • To analyze the diagnostic and prognostic significance of CHAF1B in LUAD.
  • To explore CHAF1B's association with immune infiltration, immunological checkpoints, and drug sensitivity in LUAD.

Main Methods:

  • Utilized GEPIA2, UALCAN, and TCGA databases for expression and survival analysis.
  • Performed Gene Ontology (GO) enrichment and single-cell function analyses.
  • Conducted in vitro studies (qRT-PCR, CCK8, Transwell, glucose/lactate metabolism) and predicted regulatory pathways (lncRNAs-miRNA-CHAF1B axis).

Main Results:

  • CHAF1B expression was examined in LUAD tissues.
  • CHAF1B's association with drug sensitivity, immune cell infiltration, and immunological checkpoints was analyzed.
  • In vitro studies confirmed CHAF1B's role in LUAD proliferation, migration, invasion, and glycolysis; suppression of CHAF1B decreased these processes.

Conclusions:

  • CHAF1B may serve as an innovative biomarker for LUAD diagnosis and prognosis.
  • Targeting CHAF1B could be a potential therapeutic strategy for LUAD.
  • Further research into the lncRNAs-miRNA-CHAF1B axis may elucidate molecular mechanisms in LUAD.