Related Experiment Video

Updated: Jun 2, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.5K

miR-507 Acts as a Tumor Suppressor in Renal Cell Carcinoma Cells by Targeting STEAP3

Gong Xiaobo1, Huang Jian1, Guo Linjie1

  • 1Department of Urology, The First Affiliated Hospital of Yangtze University, Jingzhou, 434000, Hubei Province, P.R. China.

Combinatorial Chemistry & High Throughput Screening
|January 14, 2025
PubMed
Abstract

Insights

MicroRNA-507 (miR-507) acts as a tumor suppressor in renal cell carcinoma (RCC) by inhibiting STEAP3 expression. This finding suggests miR-507 as a potential biomarker for RCC surveillance and treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal cell carcinoma (RCC) incidence is rising, with metastatic disease causing significant mortality.
  • MicroRNAs (miRNAs) play a role in RCC development, but their specific functions are not fully understood.
  • This study investigates the role of miR-507 in RCC and its downstream target, STEAP3.

Purpose of the Study:

  • To explore the function of miR-507 in renal cell carcinoma (RCC).
  • To identify STEAP3 as a direct downstream target of miR-507.
  • To elucidate the molecular mechanism by which miR-507 affects RCC progression.

Main Methods:

  • Bioinformatics analysis of miR-507 and STEAP3 expression in RCC tissues.
  • Cell viability (CCK-8), migration (Transwell), and apoptosis (flow cytometry) assays.
  • Luciferase reporter assay to confirm miR-507 targeting of STEAP3.
  • Western blotting to analyze protein expression levels (STEAP3, p53, xCT).

Main Results:

  • Low miR-507 expression in RCC tissues correlates with poor survival.
  • STEAP3 is significantly upregulated in RCC and is targeted by miR-507.
  • Overexpression of miR-507 suppresses cell viability, migration, and induces apoptosis by downregulating STEAP3 via the p53/xCT pathway.

Conclusions:

  • miR-507 acts as a tumor suppressor in RCC by modulating the STEAP3/p53/xCT axis.
  • miR-507 demonstrates potential as a biomarker for RCC surveillance and therapeutic strategies.
Keywords:
MiR-507STEAP3invasion.metastasisproliferationrenal cell carcinoma

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.3K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice00:07

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.2K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.7K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.4K