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Heat shock protein 70 levels in children with nephrotic syndrome

Bağdagül Aksu1, Zeynep Nagehan Yürük Yıldırım2, Asuman Gedikbaşı1

  • 1Department of Pediatric Basic Sciences, Institute of Child Health, İstanbul University, İstanbul, Türkiye.

The Turkish Journal of Pediatrics
|January 14, 2025
PubMed

Insights

Urine heat shock protein 70 (uHSP70) levels are elevated in children with steroid-sensitive nephrotic syndrome (SSNS) before treatment and decrease with prednisolone therapy, indicating reduced renal stress. uHSP70 may serve as a biomarker for monitoring treatment response.

Area of Science:

  • Pediatric Nephrology
  • Biomarker Discovery
  • Glomerular Diseases

Background:

  • Idiopathic nephrotic syndrome (NS) is a common glomerular disease in children.
  • Heat shock protein 70 (HSP70) responds to cellular stress, including infections and oxidative stress.
  • Investigating HSP70 in steroid-sensitive nephrotic syndrome (SSNS) can offer insights into disease mechanisms and treatment response.

Purpose of the Study:

  • To evaluate serum and urine HSP70 levels in children with SSNS.
  • To assess changes in HSP70 levels during prednisolone treatment.
  • To determine if HSP70 levels can differentiate between frequently and infrequently relapsing SSNS.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to measure serum and urine HSP70 levels.
  • Samples were collected from 36 SSNS patients and 35 healthy children at four time points: before treatment and on days 15, 30, and 90.
  • HSP70 levels were analyzed in relation to treatment status and relapse frequency.

Main Results:

  • Pre-treatment urine HSP70 (uHSP70) and uHSP70/creatinine ratios were significantly higher in SSNS patients compared to controls (p<0.0001).
  • Serum HSP70 (sHSP70) levels were lower in SSNS patients before treatment (p=0.002).
  • uHSP70 levels significantly decreased during prednisolone treatment (p<0.0001), while no significant differences in HSP70 levels were observed between frequent and infrequent relapsers.

Conclusions:

  • Elevated uHSP70 in SSNS patients before treatment suggests renal stress and damage.
  • Decreasing uHSP70 levels with prednisolone therapy indicate reduced renal stress and effective treatment.
  • uHSP70 shows potential as a biomarker for monitoring renal damage and treatment response in SSNS, but does not differentiate relapse frequency.
Abstract

Keywords:
HSP70heat shock protein 70pediatricprednisolonerelapsesteroid-sensitive nephrotic syndrome

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