Characterization of Enterobacterales growing on selective CPE screening plates with a focus on non-carbapenemase-producing strains
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View abstract on PubMed
Summary
This summary is machine-generated.Most bacteria growing on Carbapenemase-producing Enterobacterales (CPE) screening plates are not CPE but are multidrug-resistant (MDR). Reporting these MDR non-CPE isolates could aid treatment and infection control efforts.
Area Of Science
- Clinical Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background
- Selective screening plates are crucial for detecting Carbapenemase-producing Enterobacterales (CPE) carriers to prevent healthcare-associated infections.
- These plates are designed to isolate CPE, but non-CPE bacteria can also grow, and their characterization is often overlooked.
- Understanding the prevalence and characteristics of non-CPE isolates on CPE selective media is essential for comprehensive infection control.
Purpose Of The Study
- To characterize the non-carbapenemase-producing Enterobacterales (non-CPE) that grow on selective CPE screening plates.
- To determine the antibiotic susceptibility profiles and resistance mechanisms of these non-CPE isolates.
- To assess the clinical relevance of reporting non-CPE isolates detected during routine CPE screening.
Main Methods
- Isolates were collected from two Israeli medical institutions over a six-month period.
- Species identification and antibiotic susceptibility testing were performed using VITEK systems, with meropenem MICs determined by E-test.
- Fourier-transform infrared spectroscopy (FTIR) and whole genome sequencing were employed for phenotypic and genotypic characterization of key isolates.
Main Results
- 60.4% of isolates growing on CPE selective plates were non-CPE, predominantly Klebsiella pneumoniae and Escherichia coli.
- A significant proportion (78.2%) of non-CP K. pneumoniae and E. coli were susceptible to meropenem, but all were multidrug-resistant (MDR).
- Sequenced non-CPE isolates exhibited multiple resistance mechanisms, including various beta-lactamases, penicillin-binding protein modifications, and porin mutations.
Conclusions
- The majority of isolates recovered from CPE selective screening plates are non-CPE but possess multidrug resistance.
- These MDR non-CPE isolates, though not the primary target, represent a significant bacterial population with potential clinical implications.
- Laboratory reporting of MDR non-CPE isolates could enhance treatment guidance, prophylaxis strategies, and overall infection control measures.
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