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A mouse coccygeal intervertebral disc degeneration model with tail-looping constructed using a suturing method.

Wei Xie1, Zemao Huang1,2, Ziyang Huang1

  • 1Department of Orthopaedic Surgery, The 909th Hospital, School of Medicine, Xiamen University, Zhangzhou, China.

Animal Models and Experimental Medicine
|January 14, 2025
PubMed
Summary

Researchers developed a novel surgical method to create an animal model of intervertebral disc degeneration (IDD). This effective model aids in understanding the causes and treatments for degenerative disc disease.

Keywords:
animal modelintervertebral disc degenerationmechanical stressmice

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Area of Science:

  • Biomedical Engineering
  • Orthopedics
  • Regenerative Medicine

Background:

  • Intervertebral disc degeneration (IDD) is a prevalent degenerative condition.
  • Ethical limitations in human research necessitate animal models for IDD studies.
  • Understanding IDD pathogenesis and treatment requires robust experimental models.

Purpose of the Study:

  • To establish and validate a novel surgical model of intervertebral disc degeneration (IDD) in mice.
  • To assess the efficacy of a new surgical technique for inducing IDD.
  • To provide a reliable animal model for future IDD research.

Main Methods:

  • A surgical technique involving tail flexion and suturing was employed to create a coccygeal IDD model in 30 mice.
  • Microcomputed tomography (μCT) and magnetic resonance imaging (MRI) were used to assess disc height and signal intensity.
  • Histological analyses included Hematoxylin and Eosin (HE), Safranin O-Fast Green, and immunohistochemical staining for tissue morphology and extracellular matrix evaluation.

Main Results:

  • The surgical procedure was successful in inducing IDD in the mouse coccygeal spine.
  • The model demonstrated no significant post-operative complications.
  • Varying degrees of IDD severity could be achieved by adjusting the bending angle, indicating model controllability.

Conclusions:

  • The developed surgical method provides an effective and reproducible animal model for studying intervertebral disc degeneration.
  • This model facilitates research into the etiology and potential therapeutic strategies for degenerative disc disease.