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Visual detection of kanamycin with functionalized Au nanoparticles.

Xigui Liu1, Danlian Huang2, Cui Lai2

  • 1Institute of Chemical Engineering, Guangdong Academy of Sciences, Guangzhou, 510665, People's Republic of China. liuxigui24@126.com.

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Summary

A new colorimetric method uses 6-thioguanine functionalized gold nanoparticles to detect kanamycin. This simple, rapid, and selective strategy offers sensitive detection of kanamycin in real samples.

Keywords:
6-thioguanineAu nanoparticlesColorimetric strategyHydrogen bond interactionKanamycin

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Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Environmental Science

Background:

  • Accurate detection of kanamycin is crucial for pharmaceutical quality control and environmental monitoring.
  • Existing methods for kanamycin detection can be complex, time-consuming, or require expensive instrumentation.
  • Development of simple, sensitive, and selective detection strategies is needed.

Purpose of the Study:

  • To develop a simple, rapid, and highly selective colorimetric method for kanamycin detection.
  • To utilize hydrogen bond interactions between 6-thioguanine functionalized gold nanoparticles and kanamycin for detection.
  • To quantify kanamycin concentrations using UV-vis spectrophotometry.

Main Methods:

  • Functionalization of gold nanoparticles (AuNPs) with 6-thioguanine (6-TG).
  • Exploitation of hydrogen bonding between 6-TG and kanamycin to induce AuNP aggregation.
  • Colorimetric detection of AuNP aggregation via UV-vis spectrophotometry, monitoring absorbance ratios (A620/A520).

Main Results:

  • A linear relationship was observed between kanamycin concentrations (0.005 to 18 µM) and the absorbance ratio (A620/A520).
  • The method achieved a low limit of detection (LOD) of 1.8 nM and a limit of quantification (LOQ) of 5.9 nM.
  • High selectivity was demonstrated against common interfering substances, and results in real samples correlated well with HPLC.

Conclusions:

  • The developed colorimetric strategy offers a facile, inexpensive, and sensitive method for kanamycin detection.
  • The method's simplicity and lack of need for complex procedures make it suitable for practical applications.
  • This work contributes to the advancement of colorimetric detection methods for organic contaminants.