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Updated: Jun 2, 2025
09:33
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
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Poly ADP-ribosylation regulates Arc expression and promotes adaptive stress-coping
Eliyahu Dahan1, Leah Pergamenshik1, Tze'ela Taub1
1Department of Molecular Biology, Ariel University, Ariel, Israel.
Psychopharmacology
|January 14, 2025
Summary
Poly ADP-ribosylation (PARylation) is crucial for stress-coping. Inhibiting Parp1 impairs this ability by affecting gene expression, highlighting PARylation
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Acute stress response and coping strategies are vital for survival.
- The molecular mechanisms underlying stress-coping are not fully understood.
Purpose of the Study:
- Investigate the role of poly ADP-ribosylation (PARylation) in stress-coping.
- Identify genes affected by Parp1-induced histone PARylation during stress.
Main Methods:
- Mice underwent a forced swim test to assess stress response.
- Cortical PARylation and gene expression were evaluated.
- Parp1 was inhibited using ABT888 (Veliparib) to observe behavioral and molecular effects.
Main Results:
- Forced swim test increased cortical PARylation and gene expression.
- Parp1 inhibition impaired stress-coping behavior and reduced immobility.
- Inhibition decreased chromatin PARylation, histone H4 acetylation at the Arc promoter, and Arc expression.
Conclusions:
- Chromatin PARylation regulates Arc gene expression via histone acetylation.
- PARylation influences stress-coping behavior following acute stress.
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