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Updated: Jun 2, 2025

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis
Published on: December 8, 2023
Advanced applications of two-dimensional liquid chromatography in quantitative analysis of natural products
Yang Lu1, Qiubing Qin1, Juan Pan1
1College of Pharmacy, Shenzhen Technology University, Shenzhen, China.
Abstract:
Two-dimensional liquid chromatography (2D-LC) separation systems, based on two independent columns with different separation mechanisms, have exhibited strong resolving power for complex samples. Therefore, in recent years, the exceptional resolution of 2D-LC has significantly advanced the chemical separation of natural products, such as complex herbs, greatly facilitating their qualitative and quantitative analysis. This paper aims to review the latest strategies of 2D-LC in the quantitative analysis of complex chemical compositions in natural products. To this end, the major advantages and disadvantages of various column couplings in 2D-LC are discussed based on specific studies, along with suggested solutions to address the identified drawbacks. Moreover, the applications of different detectors combined with the latest chemometrics in 2D-LC for accurate quantitative analysis of natural products are reviewed and discussed.
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