Structural characteristics of polysaccharide isolated from Potentilla anserina L. and its mitigating effect on Zearalenone-induced oxidative stress in Sertoli cells
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.This study characterizes a natural polysaccharide, PAP-1b from Potentilla anserina L. roots, revealing its antioxidant properties by protecting porcine Sertoli cells from oxidative stress.
Area Of Science
- Natural Product Chemistry
- Cell Biology
- Biochemistry
Background
- Oxidative stress is implicated in various cellular dysfunctions.
- Natural polysaccharides are explored for their therapeutic potential.
- Potentilla anserina L. is a plant with traditional medicinal uses.
Purpose Of The Study
- To structurally characterize the polysaccharide PAP-1b from Potentilla anserina L. roots.
- To evaluate the antioxidant activity of PAP-1b in a cellular model of oxidative stress.
Main Methods
- Structural elucidation of PAP-1b using methylation and NMR analyses.
- Assessment of PAP-1b's antioxidant effects on zearalenone-induced oxidative stress in porcine Sertoli cells.
- Measurement of cell viability, antioxidant enzyme activities, reactive oxygen species (ROS), lactate dehydrogenase (LDH), malondialdehyde (MDA), mitochondrial membrane potential, and apoptosis rate.
Main Results
- PAP-1b, with a molecular weight of 1.22 × 10^4 Da, is composed mainly of glucose and galactose with specific glycosidic linkages.
- PAP-1b significantly protected porcine Sertoli cells against zearalenone-induced oxidative stress.
- PAP-1b demonstrated antioxidant effects by increasing cell viability, enhancing antioxidant enzymes, and reducing oxidative damage markers.
Conclusions
- PAP-1b exhibits significant antioxidant activity, particularly through the mitochondrial pathway.
- The findings suggest that Potentilla anserina L. polysaccharides are promising candidates for functional food applications.
- PAP-1b effectively mitigates oxidative stress in a cellular model, highlighting its potential health benefits.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.