Related Experiment Video
Updated: Jun 2, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
Manganese Peroxidase Participates in the Liquid-Solid-Gas Triphase Regulation on Microbial Degradation of
Lei Zhu1, Juan Ding1, Wenjing Xue1
1Department of Microbiology, Sanya Institute of Nanjing Agricultural University, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Abstract:
The three-phase structure of solid-state fermentation (SSF) directly affects substrate degradation and fermentation efficiency. However, the mechanism of three-phase regulation on lignocellulose utilization and microbial metabolism is still unclear. Based on comparative transcriptome analysis, a lignocellulose degrading enzyme, manganese peroxidase (GlMnP), which was significantly affected by water stress meanwhile related to triphase utilization, was screened to reveal the mechanism using Ganoderma lucidum as the reference strain. The results showed that GlMnP directly participates in lignocellulose degradation by positively regulating the activity of carboxymethylcellulase (CMCase), filter paper (FPAse), and laccase (LACase) enzymes, and indirectly participates in lignocellulose degradation by negatively regulating the redox levels in microorganisms. In addition, GlMnP can also control microbial glycolysis rate to enhance lignocellulose utilization. The results indicated that GlMnP participates in the liquid-solid-gas triphase regulation on lignocellulose degradation by G. lucidum in SSF.
More Related Videos
16:33Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
08:21Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022