Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Complex Ions03:45

Formation of Complex Ions

23.2K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
23.2K
Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

887
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
887
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

20.5K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
20.5K
Complexation Equilibria: Factors Influencing Stability of Complexes01:09

Complexation Equilibria: Factors Influencing Stability of Complexes

324
In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
324
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

464
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
464
Complexation Equilibria: The Chelate Effect01:19

Complexation Equilibria: The Chelate Effect

432
In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
432

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrids of Benzenesulfonamide Oxadiazole Derivatives with Dual CA II and COX-2 Inhibitory Activity Demonstrating Antiglaucoma and Anti-inflammatory Action: Synthesis, In Silico Insights, and In Vitro and In Vivo Bioevaluation.

Journal of medicinal chemistry·2026
Same author

Engineering an Extremely Hybrid PKS for Adipic Acid Production.

ACS synthetic biology·2026
Same author

Metadensity Functional Learning for Classical Fluids: Regularizing with Pair Correlations.

The journal of physical chemistry. B·2026
Same author

Dual FLT3/MAPK14 Proteolysis-Targeting Chimera (PROTAC) Induces Potent Acute Myeloid Leukemia Cell Death.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Dual Modality and Site-differentiated Sentinel Node Mapping in Vulvar Cancer.

Anticancer research·2026
Same author

Correction: The necessity of multi-parameter normalization in cyanobacterial research: A case study of the PsbU in Synechocystis sp. PCC 6803 using CRISPRi.

The Journal of biological chemistry·2026

Related Experiment Video

Updated: Jun 2, 2025

Dynamic Electrochemical Measurement of Chloride Ions
07:32

Dynamic Electrochemical Measurement of Chloride Ions

Published on: February 5, 2016

11.4K

Leveraging Metal Complexes for Microsecond Lifetime-Based Chloride Sensing.

Jared Morse1, Nnamdi Ofodum1, Fung Kit Tang1

  • 1Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, New York 13676, United States.

ACS Sensors
|January 15, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel iridium(III) complex for sensitive and selective chloride sensing. This new luminophore overcomes limitations of existing indicators, enabling robust cellular imaging regardless of pH changes.

Keywords:
Chloridechloride detectionchloride-sensitive luminophoreiridium complexlong lifetime luminescence

More Related Videos

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride
07:23

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride

Published on: July 1, 2020

14.0K
Detection and Recovery of Palladium, Gold and Cobalt Metals from the Urban Mine Using Novel Sensors/Adsorbents Designated with Nanoscale Wagon-wheel-shaped Pores
10:31

Detection and Recovery of Palladium, Gold and Cobalt Metals from the Urban Mine Using Novel Sensors/Adsorbents Designated with Nanoscale Wagon-wheel-shaped Pores

Published on: December 6, 2015

28.0K

Related Experiment Videos

Last Updated: Jun 2, 2025

Dynamic Electrochemical Measurement of Chloride Ions
07:32

Dynamic Electrochemical Measurement of Chloride Ions

Published on: February 5, 2016

11.4K
Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride
07:23

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride

Published on: July 1, 2020

14.0K
Detection and Recovery of Palladium, Gold and Cobalt Metals from the Urban Mine Using Novel Sensors/Adsorbents Designated with Nanoscale Wagon-wheel-shaped Pores
10:31

Detection and Recovery of Palladium, Gold and Cobalt Metals from the Urban Mine Using Novel Sensors/Adsorbents Designated with Nanoscale Wagon-wheel-shaped Pores

Published on: December 6, 2015

28.0K

Area of Science:

  • Coordination Chemistry
  • Chemical Sensing
  • Bioimaging

Background:

  • Chloride ions are crucial for cellular homeostasis, but existing indicators have limitations like pH sensitivity and short lifetimes.
  • These limitations hinder accurate chloride detection and imaging in biological systems.
  • There is a need for robust chloride sensors applicable in diverse physiological environments.

Purpose of the Study:

  • To develop a novel, sensitive, and selective chloride indicator.
  • To investigate the phosphorescence properties of a new iridium(III) complex for chloride sensing.
  • To demonstrate the utility of the developed sensor for cellular chloride imaging.

Main Methods:

  • Synthesis of an iridium(III) complex featuring a pyridinium recognition unit for chloride.
  • Characterization of the complex's phosphorescence emission properties, including sensitivity, selectivity, and lifetime.
  • Application of the complex for imaging chloride ions in live and fixed cells.

Main Results:

  • The synthesized iridium(III) complex (1) demonstrated high sensitivity and selectivity for chloride sensing across various physiological conditions (pH, ionic strength).
  • The complex exhibited a microsecond emission lifetime, suitable for phosphorescence-based detection.
  • The analogue (1b) was successfully employed for dose-dependent imaging of chloride in cellular environments.

Conclusions:

  • The novel iridium(III) complex offers a promising alternative to existing chloride indicators.
  • Its robust performance and long emission lifetime facilitate reliable chloride sensing and imaging.
  • The developed sensor provides a new avenue for studying chloride dynamics in biological systems.