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Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Updated: Jun 2, 2025

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Customizable large-scale HPLC fraction collection using low-cost 3D printing.

William J Crandall1, Marco Caputo2, Lewis Marquez1,3

  • 1Molecular and Systems Pharmacology Program, Emory University, Atlanta, GA, USA.

Hardwarex
|January 15, 2025
PubMed
Summary

Researchers developed a low-cost, customizable fraction collector for high-performance liquid chromatography (HPLC) systems. This 3D-printed device enhances reproducibility and accessibility for scalable fraction collection.

Keywords:
3D PrintingFraction CollectorHPLCLow-CostSample Collection

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chemical Engineering

Background:

  • High-performance liquid chromatography (HPLC) is a crucial technique for molecular separation.
  • Automated fraction collection systems improve reproducibility but are often costly and inflexible.
  • Existing commercial fraction collectors present significant barriers to accessibility and customization for researchers.

Purpose of the Study:

  • To present the efficient construction of an affordable and customizable fraction collector for HPLC systems.
  • To overcome the limitations of commercial fraction collectors, including cost, customization, and volume capacity.
  • To provide a platform that enhances the reproducibility of scalable and iterative fraction collection methods.

Main Methods:

  • Utilized a hobbyist-grade 3D printer (Creality Ender 3 Pro) and aluminum extrusions for construction.
  • Developed a graphical user interface (GUI) for user-friendly programming of collection methods without coding.
  • Designed the system to be compatible with various HPLC systems and support large collection volumes.

Main Results:

  • Successfully constructed a low-cost (<$280 USD) customizable HPLC fraction collector.
  • Demonstrated support for significantly larger collection volumes (up to 470 mL) compared to commercial alternatives.
  • The GUI enables easy operation, requiring no prior coding experience for method programming.

Conclusions:

  • The developed 3D-printed fraction collector offers a highly customizable and cost-effective solution for HPLC applications.
  • This platform removes cost barriers and increases accessibility for researchers needing scalable fraction collection.
  • The system enhances the reproducibility of preparatory-scale fraction collection methods.