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Updated: Jun 2, 2025

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

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Mast cell-mediated microRNA functioning in immune regulation and disease pathophysiology

Qiuping Deng1, Xiuju Yao2, Siyun Fang1

  • 1Department of Clinical Laboratory, Chengdu Jinjiang Hospital for Women's and Children's Health, Chengdu, 610016, Sichuan, China.

Clinical and Experimental Medicine
|January 15, 2025
PubMed

Insights

Mast cells (MCs) release mediators influencing inflammation and tumors. MicroRNAs (miRNAs) within MCs and their extracellular vesicles (EVs) regulate MC function and impact various diseases, offering therapeutic insights.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Mast cells (MCs) are key immune cells involved in allergic reactions and the tumor microenvironment (TME).
  • MCs release various mediators, including cytokines and chemokines, upon activation, contributing to inflammation.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post-transcriptionally.

Purpose of the Study:

  • To review the role of miRNAs produced by mast cells in regulating MC activity.
  • To discuss the influence of extracellular miRNAs on mast cell function.
  • To explore the involvement of mast cell-derived miRNAs in various pathologies, including cancer.

Main Methods:

  • Literature review of studies on mast cells, miRNAs, and extracellular vesicles.
  • Analysis of the regulatory functions of miRNAs in mast cell proliferation, maturation, apoptosis, and activation.
  • Examination of the role of mast cell-derived miRNAs in intercellular communication and disease pathogenesis.

Main Results:

  • miRNAs are crucial regulators of mast cell functions.
  • Mast cells secrete miRNAs within extracellular vesicles (EVs), mediating intercellular communication.
  • MC-derived miRNAs impact diseases such as allergic conditions, neuroinflammation, and tumors.

Conclusions:

  • miRNAs produced by mast cells play a significant role in controlling MC activity and function.
  • Extracellular miRNAs, particularly those within EVs, are important mediators of mast cell-related pathologies.
  • Understanding miRNA regulation in mast cells offers potential therapeutic targets for diverse diseases.
Keywords:
DiseaseEVsImmune regulationMast cellMicroRNA

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