Phytochemical Profile, Acute, and Subacute Toxicity of Spray-Dried Hydroethanolic Extract From Punica granatum Leaves

This study aimed to provide a comprehensive understanding of the acute and subacute safety and phytochemical profile of pomegranate leaves, aligning with the growing interest in sustainable, plant-based therapeutics. The phytochemical composition and the acute and subacute toxicity of a spray-dried hydroethanolic extract from pomegranate leaves (SDE) were investigated using experimental animal models. Utilizing UV-visible spectrophotometry and liquid chromatography-mass spectrometry (LC-MS), a diverse array of tannins and flavonoids, totaling 38 compounds, was identified. The findings revealed that SDE exhibited no adverse effects across various tests, including macroscopic, biochemical, hematological, and histological evaluations, even at high doses (2000 mg/kg for single doses and 1000 mg/kg for repeated doses). Furthermore, SDE significantly (p < 0.05) reduced serum total cholesterol levels in both acute and subacute toxicity evaluations, suggesting a positive impact on lipid metabolism. This research not only confirms the safety of pomegranate leaf spray-dried hydroethanolic extract at significant concentrations but also highlights its potential as a source of bioactive compounds with therapeutic benefits. The absence of toxicity, coupled with its cholesterol-lowering properties, supports the use of pomegranate leaves in medicinal, nutritional, and food additive applications. Additionally, this study provides essential phytochemical and safety data, paving the way for future research exploring its potential as an active ingredient.