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Updated: Jul 24, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Design of magnetic polyethyleneimine-fluorescein isothiocyanate composites toward efficient enrichment of
Jinfeng Zhang1, Yaming Zhao1, Zirui Wang1
1College of Chemistry, Jilin University, Changchun 130012, PR China.
Abstract:
Phosphoproteins maintain the normal metabolic activity of the organisms. Direct phosphopeptides detection is difficult to be realized by mass spectroscopy (MS) due to the low ionization efficiency, low abundance of phosphopeptides and interferences of complicated biological fluids. In the present work, a magnetic composite material was prepared by combining polyethyleneimine (PEI) and fluorescein isothiocyanate (FITC) focusing on phosphopeptides enrichment. Fourier-transform infrared spectroscopy, thermogravimetry analysis, X-ray photoemission spectroscopy, X-ray diffraction and UV-vis spectrometry measurement results confirmed the successful synthesis of Fe3O4@PEI@FITC material. FITC was facilely connected with PEI, and the two motifs endowed the efficient enrichment capacity of phosphopeptides by simple regulation of pH. The Fe3O4@PEI@FITC material was applied to the detection of phosphopeptides from real samples, implying that it can serve as an applicable platform for phosphoproteomics analysis.
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