Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AI-Powered Deep Visual Proteomics Reveals Critical Molecular Transitions in Pancreatic Cancer Precursors.

Cancer discovery·2026
Same author

Functional landscape of ubiquitin linkages couples K29-linked ubiquitylation to epigenome integrity.

The EMBO journal·2025
Same author

Deep visual proteomics reveals an in vivo-like phenotype of orthotopically transplanted human colon organoids.

Cell systems·2025
Same author

AI-powered Deep Visual Proteomics reveals critical molecular transitions in pancreatic cancer precursors.

bioRxiv : the preprint server for biology·2025
Same author

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression.

Cancer cell·2025
Same author

Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics.

STAR protocols·2025

Related Experiment Video

Updated: Jun 2, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.5K

Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics.

Xiang Zheng1, Andreas Mund2, Matthias Mann3

  • 1Novo Nordisk Foundation Center for Protein Research, University of Copenhagen 2200, Copenhagen, Denmark; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark.

Molecular Cell
|January 15, 2025
PubMed
Summary

This study introduces mipDVP, a novel method for analyzing tumor microenvironments. It reveals spatial immune cell patterns crucial for understanding cancer and developing new immunotherapies.

Keywords:
cancerdeep visual proteomicsimmunotherapymass spectrometrymultiplexed imagingspatial medicinespatial proteomicstumor microenvironmenttumor-infiltrating lymphocytes

More Related Videos

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.0K
Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

7.2K

Related Experiment Videos

Last Updated: Jun 2, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.5K
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.0K
Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

7.2K

Area of Science:

  • Oncology
  • Immunology
  • Proteomics

Background:

  • Understanding tumor-immune interactions is vital for cancer immunotherapy.
  • The tumor microenvironment (TME) complexity presents challenges in therapeutic development.

Purpose of the Study:

  • To introduce mipDVP, a spatial proteomic profiling approach.
  • To decipher cellular interplay within the TME of colorectal and tonsil cancers.

Main Methods:

  • mipDVP integrates highly multiplexed imaging, single-cell laser microdissection, and mass spectrometry.
  • Spatial proteomic analysis was performed on human colorectal and tonsil cancer samples.

Main Results:

  • In colorectal cancer (cold tumor), an immunosuppressive macrophage barrier was identified, hindering T-cell infiltration.
  • Distinct T-cell functional states were observed in different tumor compartments.
  • In tonsil cancer (hot tumor), proteomic heterogeneity was linked to cytotoxic T-cell proximity.
  • Tumor parenchyma T-cells showed metabolic adaptations to hypoxia.

Conclusions:

  • The mipDVP strategy provides spatially resolved insights into TME complexity.
  • This approach can identify novel immunotherapy targets and predictive biomarkers.