Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Middle-Throughput LC-MS-Based Platelet Proteomics with Minute Sample Amounts Using Semiautomated Positive Pressure

Stefan Loroch1,2,3,4, Eleftherios Panagiotidis4, Kristoffer Klewe3

  • 1Medical Proteome-Center (MPC), Medical Faculty, Ruhr-University Bochum, Bochum, Germany.

Thrombosis and Haemostasis
|January 15, 2025
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ELISA-negative HIT: antibody recognition and relevance.

Blood·2026
Same author

Experimental Models of Anti-PF4 Immunothrombosis: Mechanistic Insights, Translational Value, and Unmet Needs.

Thrombosis and haemostasis·2026
Same author

Phosphorylated toll-like receptor 4 defines a high-risk sepsis endotype.

Critical care (London, England)·2026
Same author

Preventing overfilling of blood bags to reduce clot formation and improve donor safety.

Vox sanguinis·2026
Same author

Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons.

Science advances·2026
Same author

Reducing Bacterial Contamination of Blood Bags with a Double Knot.

The New England journal of medicine·2026
This summary is machine-generated.

A new proteomics platform enables detailed platelet analysis using minimal blood samples, identifying 36 disease markers. This advancement offers a sensitive and high-throughput alternative to traditional methods for platelet research and diagnostics.

Area of Science:

  • Platelet biology and proteomics
  • Mass spectrometry-based proteomics
  • Biomarker discovery

Background:

  • Comprehensive platelet characterization requires multiple assays, often limited by sample availability.
  • Existing omics disciplines demand significant sample material, necessitating downscaling of methods.
  • Current methods for platelet analysis can require large volumes of blood, posing challenges for certain patient groups.

Purpose of the Study:

  • To develop a novel sample preparation platform for proteomics analysis of limited platelet samples.
  • To enhance sensitivity, reproducibility, and throughput for platelet proteomics.
  • To identify clinically relevant platelet disease markers using minimal sample input.

Main Methods:

  • Development of a 384-well format sample preparation platform for proteomics.

Related Experiment Videos

  • Utilized only 3 μg of purified platelet protein (approx. 2 x 10^6 platelets).
  • Liquid chromatography-mass spectrometry (LC-MS)-based proteomics analysis.

    • Main Results:

    • Successfully detected key players in platelet activation cascades.
    • Identified 36 clinically relevant platelet disease markers with a non-state-of-the-art instrument.
    • Achieved a >2-fold increase in sensitivity, improved reproducibility, and 4-fold increase in throughput compared to previous methods.

    Conclusions:

    • The novel platform enables sensitive LC-MS-based platelet proteomics from minimal blood volumes.
    • This approach is a powerful alternative to conventional assays, immunoaffinity, and genomic methods.
    • The platform has significant potential for rapid platelet phenotyping in research and diagnostics.