Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methods for in vitro percutaneous absorption studies IV: The flow-through diffusion cell.

R L Bronaugh, R F Stewart

    Journal of Pharmaceutical Sciences
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessment of skin absorption and irritation potential of arachidonic acid and glyceryl arachidonate using in vitro diffusion cell techniques.

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2007
    Same author

    In vitro human skin penetration of diethanolamine.

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2004
    Same author

    In vitro and in vivo percutaneous absorption of catechol.

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2003
    Same author

    In vitro percutaneous absorption models.

    Annals of the New York Academy of Sciences·2000
    Same author

    Percutaneous penetration and metabolism of 2-nitro-p-phenylenediamine in human and fuzzy rat skin.

    Toxicology and applied pharmacology·2000
    Same author

    The effects of an alpha hydroxy acid (glycolic acid) on hairless guinea pig skin permeability.

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·1999

    A novel flow-through diffusion cell system effectively measures percutaneous absorption. This system shows comparable results to static cells for various compounds, enhancing hydrophobic drug delivery research.

    Area of Science:

    • Pharmacology
    • Biomedical Engineering
    • Materials Science

    Background:

    • Percutaneous absorption studies are crucial for drug development.
    • Existing static diffusion cells have limitations in accurately measuring absorption dynamics.
    • A need exists for improved in vitro models that mimic in vivo conditions more closely.

    Purpose of the Study:

    • To develop and validate a novel flow-through diffusion cell system for percutaneous absorption studies.
    • To compare the performance of the flow-through system with traditional static diffusion cells.
    • To investigate the influence of receptor solution and formulation on the absorption of model compounds.

    Main Methods:

    • Development of a Teflon-based flow-through diffusion cell with a viewing window.

    Related Experiment Videos

  • Determination of optimal flow rate (≥5 mL/h) for the 0.4 mL receptor volume.
  • Comparative studies of water, cortisone, and benzoic acid permeation using flow-through and static cells.
  • In vivo and in vitro (flow-through and static cells) assessment of cortisone and benzoic acid absorption in rats.
  • Evaluation of hydrophobic compound (cinnamyl anthranilate) absorption using different receptor solutions.
  • Optimization of in vitro absorption using polyethylene glycol 20 oleyl ether.

    • Main Results:

    • The flow-through diffusion cell system demonstrated reliable performance for percutaneous absorption studies.
    • Similar absorption profiles and quantitative values were observed between flow-through and static cells for water, cortisone, and benzoic acid.
    • Good agreement was found between in vivo and in vitro results for cortisone and benzoic acid.
    • Enhanced percutaneous absorption of a hydrophobic compound was observed in the flow-through cell with normal saline.
    • Optimal in vitro absorption for all compounds was achieved using a 6% polyethylene glycol 20 oleyl ether solution.

    Conclusions:

    • The developed flow-through diffusion cell system is a valid and reliable tool for in vitro percutaneous absorption studies.
    • This system offers advantages in studying hydrophobic compounds and can provide results comparable to in vivo studies.
    • The system's performance can be further optimized using appropriate nonionic surfactants in the receptor solution.