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Published on: April 2, 2015
Structural modification of whey protein isolate via electrostatic complexation with Tremella polysaccharides and its
Yongqin Ma1, Huixue Sun1, Shanshan Zhang1
1School of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China; Scientific Research Base of Edible Mushroom Processing Technology Integration of Ministry of Agriculture and Rural Affairs, Changchun 130118, China.
Abstract:
Emulsions play an important role in food systems by encapsulating and delivering active compounds, but maintaining their stability under various conditions can be challenging. This study explored how the concentrations of Tremella polysaccharides (TPs) (0-0.75 %) affects the structural of whey protein isolate (WPI) and the stability of their emulsions at pH 4.5. At this pH, electrostatic interactions between WPI and TPs exposed hydrophobic groups within the protein, increased β-sheet contents, and improved the hydrophilic-hydrophobic balance, which enhanced emulsifying performance. WPI-TPs complexes (WTS) showed a high emulsifying activity index (57.85 m2/g) and emulsion stability index (82.03 %). Compared to WPI-only emulsions, WTS emulsions had smaller particle sizes, lower Turbiscan Stability Index (TSI) values, and higher viscoelasticity, thermal stability, freeze-thaw stability, and re-emulsification capacity. Importantly, when the TPs concentration in WTS emulsions exceeded 0.375 %, the TSI value dropped below 1, showing no particle migration or peak thickness, indicating full emulsion stability. These findings suggest that TPs help stabilize WPI emulsions near their isoelectric point (pH 4.5) and offer a promising approach to improving WPI functionality in acidic environments. The WTS system provides a reliable way to stabilize emulsions under acidic conditions, supporting the development of natural, stable emulsifiers for food applications.
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