Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chlorhexidine 0.02% or polyhexamethylene biguanide 0.02% as the main treatment for Acanthamoeba keratitis.

Acta ophthalmologica·2026
Same author

Redefining the Entamoeba landscape with next-generation sequencing: Implications for diagnosis and epidemiology.

Advances in parasitology·2026
Same author

Clinical, Molecular, and Zoonotic Perspectives on Human Cases of Cryptosporidium sp. OTUi.

Emerging infectious diseases·2026
Same author

Travelers Returning to Denmark with Hypereosinophilia and Ancylostoma Infections Detected Using Broad-Specificity Amplicon-Based Next-Generation Sequencing.

The American journal of tropical medicine and hygiene·2026
Same author

Toward ethical human microbiome research: improving health through radical interdisciplinary and intercultural co-laboration.

Microbiome·2026
Same author

Navigating Blastocystis research - challenges in the next-generation sequencing era.

Trends in parasitology·2026

Related Experiment Video

Updated: Jul 6, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.4K

Pitfalls in gut single-cell eukaryote research.

Raul Yhossef Tito Tadeo1, Christen Rune Stensvold2

  • 1Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium; Center for Microbiology, VIB, Leuven, Belgium.

Trends in Parasitology
|January 15, 2025
PubMed
Summary

Gut single-celled eukaryotes (GSCEs) impact human gut ecology, but their roles are unclear. Current research using bacterial data has limitations, necessitating improved strategies for a better understanding of GSCEs in health and disease.

Keywords:
ecologygut microbiomemicrobiotaprotozoapublic healthsingle-celled parasites

More Related Videos

Reusable Single Cell for Iterative Epigenomic Analyses
10:28

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

1.2K
Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

692

Related Experiment Videos

Last Updated: Jul 6, 2026

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.4K
Reusable Single Cell for Iterative Epigenomic Analyses
10:28

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

1.2K
Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

692

Area of Science:

  • Microbiology
  • Human Gut Ecology
  • Microbiome Research

Background:

  • Gut single-celled eukaryotes (GSCEs) are abundant in humans, yet their ecological functions remain largely unknown.
  • Existing research often relies on retrospective bacterial data analysis, limiting comprehensive understanding of GSCEs.
  • GSCEs may influence gut microbiome composition, host metabolism, and health parameters.

Purpose of the Study:

  • To critically evaluate current research strategies for studying GSCEs in the human gut.
  • To identify limitations in existing methodologies, particularly those using bacterial data.
  • To propose actionable improvements for future research on GSCEs and their role in human health and disease.

Main Methods:

  • Retrospective analysis of bacterial next-generation sequencing (NGS) data.
  • Literature review and critical assessment of research methodologies.
  • Identification of research strategy pitfalls.

Main Results:

  • Current research approaches, primarily analyzing bacterial NGS data, have significant limitations in characterizing GSCEs.
  • These limitations hinder accurate assessment of GSCEs' functions and their interactions within the gut ecosystem.
  • Potential associations between GSCEs and favorable gut microbiome features are suggested but require more robust investigation.

Conclusions:

  • There is a critical need to refine research strategies for studying gut single-celled eukaryotes.
  • Addressing methodological pitfalls is essential for advancing our understanding of GSCEs' impact on human health and disease.
  • Future research should employ more direct and comprehensive methods to elucidate the roles of GSCEs in gut ecology.