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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Jun 15, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

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Comprehensive genome-scale CRISPR knockout screening of CHO cells.

Sung Wook Shin1, Su Hyun Kim2, Aghiles Gasselin2

  • 1Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea.

Scientific Data
|January 15, 2025
PubMed
Summary

This study used CRISPR knockout screening in Chinese hamster ovary (CHO) cells to identify genes critical for cell fitness and therapeutic protein production, advancing CHO cell factory development.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Genomics

Background:

  • Chinese hamster ovary (CHO) cells are crucial for producing recombinant therapeutics.
  • Improving CHO cell productivity is essential for biopharmaceutical manufacturing.

Purpose of the Study:

  • To perform a genome-scale CRISPR knockout screen in CHO-K1 cells.
  • To identify genes influencing cell fitness and therapeutic protein production.

Main Methods:

  • Utilized a virus-free, recombinase-mediated cassette exchange platform for CRISPR screening.
  • Generated genome-wide guide RNA (gRNA) amplicon sequencing data from KO libraries.
  • Validated findings through phenotypic assessment and gRNA read count analysis.

Main Results:

  • Identified essential genes impacting CHO cell fitness.
  • Discovered functional target genes associated with enhanced therapeutic protein production.
  • Generated valuable next-generation sequencing datasets for CHO genomic function analysis.

Conclusions:

  • Developed a robust CRISPR screening method for CHO cells.
  • Provided insights into CHO cell biology for improved biomanufacturing.
  • Advanced the development of next-generation CHO cell factories.