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Updated: Jun 15, 2026
00:09
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Published on: September 4, 2019
21.6K
Comprehensive genome-scale CRISPR knockout screening of CHO cells
Sung Wook Shin1, Su Hyun Kim2, Aghiles Gasselin2
1Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea.
Scientific Data
|January 15, 2025
Summary
This study used CRISPR knockout screening in Chinese hamster ovary (CHO) cells to identify genes critical for cell fitness and therapeutic protein production, advancing CHO cell factory development.
Area of Science:
- Biotechnology
- Cell Biology
- Genomics
Background:
- Chinese hamster ovary (CHO) cells are crucial for producing recombinant therapeutics.
- Improving CHO cell productivity is essential for biopharmaceutical manufacturing.
Purpose of the Study:
- To perform a genome-scale CRISPR knockout screen in CHO-K1 cells.
- To identify genes influencing cell fitness and therapeutic protein production.
Main Methods:
- Utilized a virus-free, recombinase-mediated cassette exchange platform for CRISPR screening.
- Generated genome-wide guide RNA (gRNA) amplicon sequencing data from KO libraries.
- Validated findings through phenotypic assessment and gRNA read count analysis.
Main Results:
- Identified essential genes impacting CHO cell fitness.
- Discovered functional target genes associated with enhanced therapeutic protein production.
- Generated valuable next-generation sequencing datasets for CHO genomic function analysis.
Conclusions:
- Developed a robust CRISPR screening method for CHO cells.
- Provided insights into CHO cell biology for improved biomanufacturing.
- Advanced the development of next-generation CHO cell factories.
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