Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hemopoietic precursor cells in human peripheral blood.

E Niskanen, T Olofsson, M J Cline

    American Journal of Hematology
    |January 1, 1979
    PubMed
    Summary

    Two human peripheral blood stem cell types differentiate into granulocytes. One type, forming colonies in vitro (CFU-C), is distinct from the in vivo type (CFU-DG), which appears intermediate in differentiation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Known and less well-known comorbidities in axial spondyloarthritis: what are we overlooking? Results from the SPARTAKUS cohort.

    Scandinavian journal of rheumatology·2025
    Same author

    Persistent pain and its predictors after starting anti-tumour necrosis factor therapy in psoriatic arthritis: what is the role of inflammation control?

    Scandinavian journal of rheumatology·2023
    Same author

    Consultation prevalence among children, adolescents and young adults with pain conditions: A description of age- and gender differences.

    European journal of pain (London, England)·2019
    Same author

    Predictors of work disability after start of anti-TNF therapy in a national cohort of Swedish patients with rheumatoid arthritis: does early anti-TNF therapy bring patients back to work?

    Annals of the rheumatic diseases·2017
    Same author

    Oncogenes and Anti-oncogenes in the Evolution of Human Leukemia/Lymphoma.

    Leukemia & lymphoma·2016
    Same author

    Cerebral cortex and sub-cortex lateralization in cardiovascular regulation: correlations of BOLD fMRI and heart rate variability.

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2013

    Area of Science:

    • Hematology
    • Stem Cell Biology
    • Cell Differentiation

    Background:

    • Peripheral blood harbors stem cells crucial for granulocytic differentiation.
    • Two distinct stem cell populations, CFU-C and CFU-DG, are involved in this process.
    • These cells can be differentiated by their colony-forming abilities in vitro and in vivo.

    Purpose of the Study:

    • To characterize and differentiate between two types of human peripheral blood stem cells.
    • To investigate the role of CFU-C and CFU-DG in granulocytic differentiation.
    • To explore the potential hierarchical relationship between these stem cell populations.

    Main Methods:

    • Utilizing in vitro agar colony formation assays to identify CFU-C.
    • Employing in vivo diffusion chamber assays with plasma clots to identify CFU-DG.
    • Conducting kinetic studies to analyze the differentiation pathways.

    Main Results:

    • Human peripheral blood contains two distinct stem cell types capable of granulocytic differentiation.
    • CFU-C forms colonies in agar in vitro, while CFU-DG forms colonies in plasma clots in vivo.
    • Kinetic data indicate CFU-DG is an intermediate stage between pluripotent stem cells and CFU-C.

    Conclusions:

    • CFU-C and CFU-DG represent distinct stages in granulocytic differentiation from human peripheral blood stem cells.
    • CFU-DG serves as a crucial intermediate, bridging pluripotent stem cells and the more differentiated CFU-C.
    • This study clarifies the hierarchy of stem cell differentiation in the granulocytic lineage.

    Related Experiment Videos