Related Experiment Video For Raman mapping
Updated: Apr 28, 2026
Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
Published on: July 25, 2011
Assessment of Surgical Margin of Tongue Squamous Cell Carcinoma via Raman Mapping
Zhongxu Li1, Lili Xue2, Xiaobo Dai1
1State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Department of Head and Neck Oncology Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
Objectives:
This study introduces a novel classification approach that combines convolutional neural network (CNN) and Raman mapping to differentiate between tongue squamous cell carcinoma (TSCC) and non-tumorous tissue, as well as to identify different histological grades of TSCC.
Materials And Methods:
In this study, 240 Raman mappings data from 30 tissue samples were collected from 15 patients who had undergone surgical resection for TSCC. A total of 18,000 sub-mappings extracted from Raman mappings were then used to train and test a CNN model, which extracted feature representations that were subsequently processed through a fully connected network to perform classification tasks based on the Raman mapping data.
Results:
The experimental results indicated that the proposed method achieved competitive classification accuracy above 83%. To further validate the effectiveness of the Raman mapping, its performance was compared with Raman spectroscopy, demonstrating a competitive accuracy rate.
Conclusions:
The promising outcomes from this application of CNN in Raman mapping suggest that this technique could be a reliable method for intraoperative assessment of surgical margins, potentially leading to shorter detection times.
Related Concept Videos
01:20Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
01:26Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
01:26Assessment of the Mouth
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.