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Optical diffraction tomography using a self-reference module.

Zhengyuan Tang1, Julianna Winnik2, Bryan M Hennelly1,3

  • 1Department of Electronic Engineering, Maynooth University, Maynooth, Co. Kildare, Ireland.

Biomedical Optics Express
|January 16, 2025
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Summary
This summary is machine-generated.

We developed a novel, cost-effective optical diffraction tomography (ODT) system for label-free 3D refractive index imaging. This modular system integrates with existing microscopes and offers dual imaging modes for biological sample analysis.

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Area of Science:

  • Biomedical optics
  • Cellular imaging
  • Holography

Background:

  • Label-free imaging is crucial for studying live biological samples.
  • Current optical diffraction tomography (ODT) systems can be complex and expensive.
  • There is a need for adaptable and cost-effective ODT solutions.

Purpose of the Study:

  • To present a novel, modular, and cost-effective optical diffraction tomography (ODT) system.
  • To enable label-free, 3D refractive index (RI) imaging of biological samples.
  • To integrate ODT with existing life-science microscopes.

Main Methods:

  • A modular two-part system: illumination module with galvo-scanner and a self-reference holographic capture module.
  • Automated alignment protocol for seamless integration with standard life-science microscopes.
  • Utilized self-reference off-axis holography for data acquisition.

Main Results:

  • Demonstrated a compact, cost-effective ODT system with simple alignment.
  • Achieved label-free, 3D RI imaging capabilities.
  • Enabled switching between ODT and real-time synthetic aperture digital holographic microscopy (SA-DHM) modalities.
  • Experimental validation using a kidney cancer cell line.

Conclusions:

  • The developed modular ODT system offers a cost-effective and versatile solution for 3D RI imaging.
  • Its compatibility with standard microscopes and dual-modality feature enhance its utility in biological research.
  • This approach facilitates advanced label-free cellular analysis.