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A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
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16S rRNA amplicon sequencing of organic and conventional chickens
Anuradha J Punchihewage-Don1, Nur A Hasan2, Salina Parveen1
1Food Microbiology and Safety Laboratory, Department of Agriculture, Food and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, Maryland, USA.
Microbiology Resource Announcements
|January 17, 2025
Abstract:
We report 16S rRNA sequencing of microbiomes associated with organic and conventional retail chickens (n=31), processed via whole-carcass enrichment and rinse methodologies. Amplicon sequencing was performed targeting the V3-V4 region of the 16S rRNA gene, which resulted with Good's coverage exceeding 99.7% of the libraries.

