Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Closing the nitrogen loop in groundwater with biohybrid technologies.

Trends in biotechnology·2026

Same author

Mass Spectrometry Imaging in ACS Journals.

ACS measurement science au·2026

Same author

High-throughput Optical Analysis to Inform Design of Electrochemical Biosensors.

ACS measurement science au·2026

Same author

Data-Driven Electrochemistry Reveals the Impact of Hydrophobicity on Aptamer Cross-Reactivity.

ACS measurement science au·2026

Same author

Employing AC and DC Electrolysis to Modulate Electroenzymatic Pathways for Efficient and Stereoselective H-D Exchange.

Journal of the American Chemical Society·2026

Same author

Investigation of Gramicidin A Incorporation into Phospholipid Vesicle Bilayers Using Optical-Trapping Confocal Raman Microscopy.

Applied spectroscopy·2026

Same journal

EXPRESS: Deterministic Compressed Sensing in Time-Domain Spectroscopy.

Applied spectroscopy·2026

Same journal

EXPRESS: Multi-Parameter Wavelength Characterization of Array Spectrometers Under Near-Limit Sampling Conditions.

Applied spectroscopy·2026

Same journal

EXPRESS: A Validated Reference Database for Twentieth-Century Cd-Based Pigments: Integrated Structural and Compositional Characterization.

Applied spectroscopy·2026

Same journal

EXPRESS: Two-Trace Two-Dimensional (2T2D-COS) in the Analysis of Brain Tissue Sample Preparation Method.

Applied spectroscopy·2026

Same journal

EXPRESS: Simplified Protocol for Analyzing Polarization Properties of Scanning Tunneling Microscope (STM) Light Emission Spectra at an Oblique Angle.

Applied spectroscopy·2026

Same journal

EXPRESS: Monitoring a Polyurethane Synthesis by Fiber-Coupled Attenuated Total Reflection Fourier Transform Infrared Spectroscopy and Multivariate Curve Resolution-Alternating Least Squares.

Applied spectroscopy·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

Cooling Rate Dependent Ellipsometry Measurements to Determine the Dynamics of Thin Glassy Films

Cooling Rate Dependent Ellipsometry Measurements to Determine the Dynamics of Thin Glassy Films

Published on: January 26, 2016

Confocal Raman Microscopy for Measuring In-Situ Temperature-Dependent Structural Changes in Poly(Ethylene Oxide) Thin

Miharu Koh¹, Jay P Kitt¹, Andrew D Pendergast¹

¹Department of Chemistry, University of Utah, Salt Lake City, Utah, USA.

Applied Spectroscopy

|January 17, 2025

Summary

This summary is machine-generated.

Investigating thin polymer films reveals distinct crystalline and amorphous phases during melting and crystallization. This study highlights Raman microscopy and SMCR analysis for understanding these crucial structural changes in poly(ethylene oxide) (PEO) films.

Keywords:

Confocal Raman microscopy PEO thin films‌confocal Raman depth profiling poly(ethylene oxide) thin films polymer phase transitions self-modeling curve resolution

More Related Videos

Methods for the Self-integration of Megamolecular Biopolymers on the Drying Air-LC Interface

Methods for the Self-integration of Megamolecular Biopolymers on the Drying Air-LC Interface

Published on: April 7, 2017

Chemical Synthesis of Porous Barium Titanate Thin Film and Thermal Stabilization of Ferroelectric Phase by Porosity-Induced Strain

Chemical Synthesis of Porous Barium Titanate Thin Film and Thermal Stabilization of Ferroelectric Phase by Porosity-Induced Strain

Published on: March 27, 2018

Related Experiment Videos

Last Updated: Jun 23, 2026

Cooling Rate Dependent Ellipsometry Measurements to Determine the Dynamics of Thin Glassy Films

Cooling Rate Dependent Ellipsometry Measurements to Determine the Dynamics of Thin Glassy Films

Published on: January 26, 2016

Methods for the Self-integration of Megamolecular Biopolymers on the Drying Air-LC Interface

Methods for the Self-integration of Megamolecular Biopolymers on the Drying Air-LC Interface

Published on: April 7, 2017

Chemical Synthesis of Porous Barium Titanate Thin Film and Thermal Stabilization of Ferroelectric Phase by Porosity-Induced Strain

Chemical Synthesis of Porous Barium Titanate Thin Film and Thermal Stabilization of Ferroelectric Phase by Porosity-Induced Strain

Published on: March 27, 2018

Area of Science:

Materials Science
Polymer Science
Spectroscopy

Background:

Crystallization from the melt is vital for semi-crystalline polymer properties.
Structural analysis of thin polymer films during phase transitions is underexplored compared to bulk polymers.
Understanding these transitions is key to controlling polymer material properties.

Purpose of the Study:

To investigate temperature-dependent structural changes in poly(ethylene oxide) (PEO) thin films during melting and crystallization.
To apply in situ Raman microscopy and self-modeling curve resolution (SMCR) analysis for detailed structural insights.
To explore the potential of confocal Raman microscopy for analyzing ultra-thin polymer films.

Main Methods:

In situ Raman microscopy was employed to monitor PEO films during thermal cycling.
Self-modeling curve resolution (SMCR) analysis was used to resolve complex spectral data.
Differential scanning calorimetry (DSC) was used for comparative thermal analysis.

Main Results:

SMCR analysis identified two distinct structural phases in PEO films: ordered crystalline and disordered amorphous.
Heating caused the crystalline structure to disappear, while cooling restored it from the amorphous phase.
Raman band broadening above melting (67°C) and sharpening below crystallization (45°C) correlated with DSC measurements.

Conclusions:

In situ Raman microscopy combined with SMCR analysis is a powerful tool for studying thin polymer film structural transitions.
The methodology can analyze polymer films as thin as 2 µm, extending beyond conventional thermal analysis capabilities.
This approach provides critical insights into the melting and crystallization behavior of surface-supported polymer films.