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Published on: December 11, 2020
CeRNA profiling and the role in regulating gonadal development in gold pompano
Changcan Yang1, Yijun Zhao1, Wendong Zhao1
1MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences / Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Qingdao / Sanya, China.
This study reveals competing RNA networks in golden pompano gonadal development. Long non-coding RNA (lnc-TGFβR) may regulate testis development by sponging microRNA (oni-let-7d-1-p3).
Area of Science:
- Aquaculture
- Genomics
- Molecular Biology
Background:
- Golden pompano (Trachinotus ovatus) is a key aquaculture species.
- Delayed sexual maturity necessitates research into its gonadal development.
- Understanding competing endogenous RNA (ceRNA) networks is crucial for insights into T. ovatus reproduction.
Purpose of the Study:
- To investigate the function of ceRNAs in T. ovatus gonads.
- To elucidate the regulatory mechanisms of the ceRNA network in fish reproduction.
- To identify key regulatory molecules involved in gonadal development.
Main Methods:
- Whole transcriptome sequencing of T. ovatus testes and ovaries.
- Identification and analysis of differentially expressed RNAs (miRNAs, mRNAs, lncRNAs, circRNAs).
- Construction and enrichment analysis of a ceRNA network.
- Experimental verification using dual-luciferase reporter assays and fluorescence in situ hybridization.
Main Results:
- Identified thousands of differentially expressed RNAs, including 96 DE-miRNAs, 2,338 DE-mRNAs, 973 DE-lncRNAs, and 94 DE-circRNAs.
- Constructed a ceRNA network implicating pathways like TGF-β and GnRH signaling in reproduction.
- Highlighted potential key regulators: oni-let-7d-1-p3, PC-3p-112794_13, Rspo1, and lnc-TGFβR.
- Verified regulatory relationships between Rspo1/lnc-TGFβR and oni-let-7d-1-p3 in sertoli cells.
Conclusions:
- Hypothesized that lnc-TGFβR acts as a sponge for oni-let-7d-1-p3, regulating testis development.
- Findings enhance understanding of ncRNA roles in fish gonadal development and ceRNA regulatory mechanisms.
- Provides novel insights into gametogenesis regulation and potential applications in aquaculture.
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