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Developing an alternative medium for in-space biomanufacturing.

Hakyung Lee1, Jinjin Diao2, Yuxin Tian1,3

  • 1Washington University in St. Louis, Saint Louis, MO, USA.

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|January 17, 2025
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Summary
This summary is machine-generated.

This study introduces alternative feedstock-driven in-situ biomanufacturing (AF-ISM) to make space biomanufacturing cost-effective. Using Martian regolith, recycled plastic, and waste, researchers produced lycopene in microgravity, comparable to Earth production levels.

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Area of Science:

  • Astrobiology
  • Biotechnology
  • Materials Science

Background:

  • Long-term human space missions require sustainable, self-sufficient life support systems.
  • Current in-space biomanufacturing relies on Earth-supplied feedstocks, hindering economic viability and scalability.
  • Reducing dependence on Earth resupply is crucial for extraterrestrial habitation.

Purpose of the Study:

  • To develop and assess an alternative feedstock-driven in-situ biomanufacturing (AF-ISM) process.
  • To evaluate the utilization of novel feedstocks for biomanufacturing in space.
  • To determine the economic feasibility of AF-ISM for producing valuable compounds like lycopene.

Main Methods:

  • Investigated Martian/Lunar regolith, recycled polyethylene terephthalate (PET), and fecal waste as alternative feedstocks (AF).
  • Utilized Rhodococcus jostii PET strain S6 (RPET S6) for lycopene production.
  • Conducted experiments under simulated microgravity conditions and performed economic analysis.

Main Results:

  • RPET S6 successfully utilized regolith simulant for mineral requirements.
  • Anaerobically pretreated fecal waste enhanced RPET S6 cell growth.
  • Lycopene production in microgravity using AF was comparable to Earth-based production.
  • AF-ISM demonstrated significant cost reductions compared to conventional methods.

Conclusions:

  • AF-ISM is a viable strategy to reduce reliance on Earth-based feedstocks for in-space biomanufacturing.
  • The use of regolith, recycled PET, and waste offers a sustainable feedstock solution.
  • This approach significantly enhances the economic feasibility of establishing self-sufficient extraterrestrial habitats.