Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

5.5K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.5K
Negative Regulator Molecules01:23

Negative Regulator Molecules

35.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.1K
Abnormal Proliferation02:23

Abnormal Proliferation

4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Positive Regulator Molecules01:45

Positive Regulator Molecules

105.7K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
105.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coexpression of IL15 Promotes Effector Differentiation and Sustained Proliferative Capacity in ALPPL2-Specific Human CAR T Cells.

Cancer immunology research·2026
Same author

Facts and Hopes of Chimeric Antigen Receptor-Redirected NK T Cells.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025
Same author

Hyperleukocytosis in a neuroblastoma patient after treatment with natural killer T cells expressing a GD2-specific chimeric antigen receptor and IL-15.

Journal for immunotherapy of cancer·2025
Same author

CAR-redirected natural killer T cells demonstrate superior antitumor activity to CAR-T cells through multimodal CD1d-dependent mechanisms.

Nature cancer·2024
Same author

Unraveling the spatial organization and development of human thymocytes through integration of spatial transcriptomics and single-cell multi-omics profiling.

Nature communications·2024
Same author

A milestone method to make natural killer T cells.

Nature biotechnology·2024

Related Experiment Video

Updated: Jun 1, 2025

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

15.2K

PRDM1 Is a Key Regulator of the NKT-cell Central Memory Program and Effector Function.

Gengwen Tian1,2, Gabriel A Barragan1, Hangjin Yu1

  • 1Department of Pediatrics, Center for Advanced Innate Cell Therapy, Texas Children's Cancer and Hematology Center, Houston, Texas.

Cancer Immunology Research
|January 17, 2025
PubMed
Summary

Researchers identified PRDM1 as a key regulator of Natural Killer T cell (NKT) function. Modulating PRDM1 levels can enhance NKT-based cancer immunotherapies by improving memory differentiation and antitumor activity.

More Related Videos

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.4K
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
07:17

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

9.8K

Related Experiment Videos

Last Updated: Jun 1, 2025

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

15.2K
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.4K
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
07:17

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

9.8K

Area of Science:

  • Immunology
  • Cancer Biology
  • Genetic Engineering

Background:

  • Natural Killer T cells (NKTs) show promise for cancer immunotherapy.
  • Identifying regulators of NKT functional fitness is crucial for enhancing therapeutic efficacy.
  • Limited knowledge exists regarding genes controlling NKT therapeutic activity.

Purpose of the Study:

  • To discover novel regulators of NKT cell functional fitness.
  • To investigate the role of PRDM1 in NKT cell differentiation and effector function.
  • To explore strategies for improving NKT-based cancer immunotherapies.

Main Methods:

  • CRISPR/Cas9-based mutagenesis screen targeting 1,118 immune-related genes.
  • Transduction of NKTs and GD2.CAR NKTs with a guide RNA library.
  • In vitro co-culture challenges with leukemia and neuroblastoma cells.
  • PRDM1 knockout and knockdown validation.
  • Transcriptional, phenotypic, and functional analyses.

Main Results:

  • Enrichment of PRDM1-specific guide RNAs identified PRDM1 as a regulator.
  • PRDM1 knockout in CAR NKTs promoted memory differentiation and resistance to exhaustion but reduced cytotoxicity.
  • Short hairpin RNA-mediated PRDM1 knockdown preserved effector function and enhanced in vivo antitumor activity.
  • PRDM1 regulates both NKT memory differentiation and effector function.

Conclusions:

  • PRDM1 is a critical regulator of NKT cell memory differentiation and effector function.
  • Targeting PRDM1 offers a potential strategy to optimize NKT-based cancer immunotherapies.
  • PRDM1 modulation can be exploited to enhance the therapeutic efficacy of NKT cells in cancer treatment.