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Related Experiment Video

Updated: Jun 17, 2026

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation
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Enhancing Cardiomyocyte Purity through Lactate-Based Metabolic Selection.

Seung Ju Seo1, Yoonhee Jin2

  • 1Department of Physiology, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.

Tissue Engineering and Regenerative Medicine
|January 17, 2025
PubMed
Summary

Lactate metabolic selection effectively purifies chemically induced cardiomyocyte-like cells (CiCMs) by reducing fibroblast contamination. This method enhances CiCMs

Keywords:
CardiomyocytesDirect reprogrammingLactateMetabolic selectionPurification

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Area of Science:

  • Cardiovascular Research
  • Cellular Reprogramming
  • Metabolic Engineering

Background:

  • Direct reprogramming of fibroblasts into cardiomyocyte-like cells (CiCMs) offers potential for cardiac regeneration.
  • Contamination by non-cardiomyocytes, mainly fibroblasts, limits the effectiveness of CiCMs.
  • A metabolic selection strategy is needed to improve CiCM purity and function.

Purpose of the Study:

  • To investigate lactate as a metabolic selection agent for enriching CiCMs.
  • To assess the impact of lactate selection on CiCM viability and purity.
  • To evaluate the functional improvement of lactate-purified CiCMs.

Main Methods:

  • Fibroblasts were reprogrammed into CiCMs.
  • Cells were cultured in glucose-depleted, lactate-supplemented medium for 4 days.
  • Cell viability, cardiac marker expression, and electrophysiological function were assessed.

Main Results:

  • Lactate treatment significantly reduced non-cardiomyocyte viability while preserving CiCMs.
  • Cardiac-specific marker expression was elevated in lactate-treated CiCMs (CiCM-LAC).
  • Purified CiCMs demonstrated enhanced contractile force, contraction frequency, and drug responsiveness.

Conclusions:

  • Lactate-based metabolic selection is an effective and practical method for enriching CiCMs.
  • This approach offers a cost-effective alternative to existing purification techniques.
  • Enriched CiCMs hold promise for advancing cardiac regeneration and therapeutic applications.