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Lab-on-paper for molecular testing with USB-powered isothermal amplification and fluidic control
Hyun Pyo Kim1, Ji-Ho Park2, Min-Gon Kim3
1AI the nutrigene, 42, Changeop-Ro, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do, 13449, Republic of Korea.
Mikrochimica Acta
|January 17, 2025
Summary
A new lab-on-paper platform offers affordable, rapid molecular diagnostics for field testing. This cost-effective system enables sensitive detection of foodborne pathogens within 30 minutes, ideal for point-of-care applications.
Area of Science:
- Biotechnology
- Microfluidics
- Molecular Diagnostics
Background:
- Growing demand for accessible, low-cost molecular diagnostics in global healthcare.
- Need for rapid, field-deployable testing solutions, especially in resource-limited settings.
Purpose of the Study:
- Introduce and evaluate an innovative lab-on-paper (LOP) platform for molecular diagnostics.
- Demonstrate the platform's capability for rapid, sensitive, and cost-effective detection of microorganisms.
Main Methods:
- Development of an automated microfluidics-based LOP system integrating isothermal amplification and paper strip detection.
- Utilized USB-powered thermal management and wax valve mechanisms for process control.
- Colorimetric detection assay for foodborne pathogens.
Main Results:
- Successful detection of Vibrio vulnificus (120 CFU/mL) and Salmonella Typhimurium (60 CFU/mL) with naked-eye visualization.
- Complete assay time (amplification and detection) achieved within 30 minutes.
- Demonstrated assay cost of $5.2 USD per test, highlighting cost-effectiveness.
Conclusions:
- The LOP platform provides a rapid, sensitive, and affordable solution for molecular diagnostics.
- Its portability and ease of use make it suitable for point-of-care testing in diverse environments.
- The technology holds significant potential for infectious disease monitoring and food safety applications.
