Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distance-weighted reflectance for arbitrary source-detector geometries from a single pencil-beam Monte Carlo simulation.

Optics letters·2026
Same author

Automated Cryo-EM and Supervised Machine Learning Enable Reproducible Characterization of Extracellular Vesicles and Co-Isolating Particles.

Journal of extracellular vesicles·2026
Same author

Quantitative model for imaging single fiber reflectance spectroscopy.

Scientific reports·2026
Same author

Traceable Refractive Index Measurements of Liquids to Standardize Extracellular Vesicle Flow Cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same author

Particle size distribution and concentration of Intralipid<sup>®</sup> 20.

Journal of biomedical optics·2026
Same author

Calibration of Flow Cytometers Enables Reproducible Measurements of Extracellular Vesicle Concentrations and Reference Range Establishment.

Journal of extracellular vesicles·2025

Related Experiment Video

Updated: Jul 18, 2026

Evaluation of Capillary and Other Vessel Contribution to Macular Perfusion Density Measured with Optical Coherence Tomography Angiography
07:18

Evaluation of Capillary and Other Vessel Contribution to Macular Perfusion Density Measured with Optical Coherence Tomography Angiography

Published on: February 18, 2022

1.7K

Achieving High-Precision Attenuation Coefficient Measurement in Optical Coherence Tomography.

Linda B Neubrand1,2,3, Xavier Attendu1,2,3,4, Ton G van Leeuwen1,2,3

  • 1Department of Biomedical Engineering and Physics, Amsterdam UMC, Location University of Amsterdam, Amsterdam, The Netherlands.

Journal of Biophotonics
|January 17, 2025
PubMed
Summary

This study validates the Cramer-Rao lower bound equation for measuring tissue optical properties with Optical Coherence Tomography (OCT). Experimental results demonstrate high precision in determining attenuation coefficients, crucial for OCT imaging applications.

Keywords:
Cramér–Rao lower boundFisher matrixattenuation coefficientcurve‐fittingdepth‐resolved estimationoptical coherence tomographyprecision

More Related Videos

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy
08:22

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Published on: January 12, 2022

3.9K
Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment
04:51

Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment

Published on: March 1, 2024

925

Related Experiment Videos

Last Updated: Jul 18, 2026

Evaluation of Capillary and Other Vessel Contribution to Macular Perfusion Density Measured with Optical Coherence Tomography Angiography
07:18

Evaluation of Capillary and Other Vessel Contribution to Macular Perfusion Density Measured with Optical Coherence Tomography Angiography

Published on: February 18, 2022

1.7K
Application of Optical Coherence Tomography to a Mouse Model of Retinopathy
08:22

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Published on: January 12, 2022

3.9K
Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment
04:51

Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment

Published on: March 1, 2024

925

Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Metrology

Background:

  • Accurate measurement of optical properties, such as attenuation coefficients, is vital for quantitative analysis in Optical Coherence Tomography (OCT).
  • Existing methods for determining attenuation coefficients in OCT may lack the precision required for certain applications.
  • The Cramer-Rao lower bound (CRLB) provides a theoretical limit for the variance of an estimator, offering a benchmark for measurement precision.

Purpose of the Study:

  • To validate the analytical Cramer-Rao lower bound (CRLB) equation for determining attenuation coefficients using a 1310 nm Optical Coherence Tomography (OCT) system.
  • To experimentally confirm the precision achievable with the CRLB-guided OCT method.
  • To establish a systematic framework for high-precision OCT attenuation measurements.

Main Methods:

  • Utilized a 1310 nm Optical Coherence Tomography (OCT) system for data acquisition.
  • Employed intralipid solutions as scattering samples to mimic biological tissues.
  • Applied the analytical Cramer-Rao lower bound (CRLB) equation to determine attenuation coefficients from OCT data.
  • Quantified measurement precision by calculating the standard deviation of the attenuation coefficients.

Main Results:

  • Experimental results successfully confirmed the validity of the analytical CRLB equation for OCT attenuation coefficient determination.
  • Achieved unprecedented precision with a standard deviation below 0.01 mm⁻¹ for intralipid samples.
  • Demonstrated the feasibility of the proposed systematic framework for high-precision OCT measurements.

Conclusions:

  • The Cramer-Rao lower bound (CRLB) equation is a valid and effective tool for precise attenuation coefficient determination in OCT.
  • The developed framework enables highly accurate measurements of optical properties using OCT, advancing its quantitative capabilities.
  • This work provides a foundation for improved OCT-based diagnostics and research requiring precise optical property quantification.