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RNA Interference Approaches to Study Epidermal Cell Adhesion

Anamika Dutta1, Michele Calder1, Lina Dagnino2,3

  • 1Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|January 17, 2025
PubMed

Keywords:
Cell adhesionEpidermal squamous carcinoma cellsGene silencingKeratinocyteRNA interferencesiRNA transfection

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RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...