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Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
Published on: January 19, 2019
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PAR2 promotes malignancy in lung adenocarcinoma
Bao Wang1,2, Ming-Da Wu3, Yue-Jiao Lan3
1Tianjin Medical University Cancer Institute and Hospital, Tianjin Medical University Tianjin 300070, China.
American Journal of Translational Research
|January 17, 2025
Summary
Proteinase-activated receptor-2 (PAR2) is upregulated in lung adenocarcinoma, correlating with poor survival and lymphatic invasion. Inhibiting PAR2 enhances chemotherapy effectiveness by promoting apoptosis in lung cancer cells.
Area of Science:
- Oncology
- Molecular Biology
Background:
- Proteinase-activated receptor-2 (PAR2) is implicated in tumor malignancy.
- Its specific role in lung adenocarcinoma (LUAD) pathogenesis is not fully understood.
Purpose of the Study:
- To investigate PAR2 expression in LUAD tissues.
- To analyze the correlation between PAR2 and clinicopathological features and survival.
- To determine PAR2's role in chemoresistance and apoptosis in lung cancer cells.
Main Methods:
- Analysis of TCGA datasets.
- Western blotting and qPCR for gene and protein expression.
- Immunohistochemistry (IHC) for tissue analysis.
- In vitro studies involving PAR2 inhibition in H1299 lung cancer cells.
Main Results:
- PAR2 levels were significantly elevated in LUAD compared to normal lung tissues.
- High PAR2 expression correlated with poor differentiation and lymphatic invasion.
- Upregulated PAR2 was associated with reduced patient survival.
- PAR2 inhibition sensitized lung cancer cells to cisplatin by modulating the BAX/BCL-2 axis and inducing ER stress and apoptosis.
- PAR2 inhibition reduced cisplatin-induced ATF4 expression.
Conclusions:
- PAR2 upregulation is a marker of poor prognosis in LUAD, linked to adverse clinicopathological features.
- PAR2 plays a critical role in promoting apoptosis resistance and reducing cisplatin cytotoxicity in LUAD.
- Targeting PAR2 may represent a potential therapeutic strategy to enhance chemotherapy efficacy in lung adenocarcinoma.
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