Astragalus polysaccharide mediates lnc-GD2H to regulate proliferation and differentiation of C2C12 muscle cells under...

Astragalus polysaccharide (APS) is a bioactive component of Astragalus species that shows protective effects on C2C12 muscle cell proliferation and differentiation under hypoxic conditions. In this study, EdU staining, cell scratch testing, quantitative reverse-transcription polymerase chain reaction, Western blotting, immunofluorescence analysis, and lnc-GD2H silencing were used to investigated the protective effects and mechanisms of action of APS against CoCl₂-induced hypoxic injury of muscle cells. Our results showed that APS promoted cell proliferation and increased the expression of lnc-GD2H, c-Myc, and Ki-67. In addition, APS protected against the effect of CoCl₂ on differentiation and increased the levels of Myog and MyHC expression. Silencing lnc-GD2H attenuated the protective effects of APS outlined above. Considering that APS may mediate the regulation of proliferation and differentiation by lnc-GD2H in C2C12 cells, and alleviates hypoxic injury induced by CoCl₂.