A comparative study on the antioxidant and antiglycation properties of different vitamin D forms

Vitamin D plays multiple roles in the body. Recently, there has been an increase in its popularity and growing interest in vitamin D supplementation. However, the mechanisms of vitamin D action have not yet been sufficiently explored. Our study focused on the antioxidant and antiglycation properties of the four primary forms of vitamin D such as cholecalciferol, calcifediol, alfacalcidol, and calcitriol. For this purpose, we used an in vitro bovine serum albumin model. Glucose, fructose, ribose, galactose, glyoxal, and methylglyoxal were glycation factors. The antiglycative mechanism of vitamin D was evaluated through in silico docking. We showed that all forms of vitamin D exhibit antioxidant and antiglycation activity, although calcitriol demonstrated the most potent effect. We observed decreased levels of advanced glycation end products and advanced oxidation protein products in samples with the addition of different vitamin D forms compared to positive control. Notably, the antioxidant and antiglycation activity is similar to routinely used antioxidants (reduced glutathione) and protein glycation inhibitors (aminoguanidine). Molecular docking analyses revealed that calcitriol demonstrated strong binding affinities with human and bovine serum albumin forming polar contacts with lysine residues highly susceptible to glycation. Calcitriol also exhibited significant interactions with the receptor for advanced glycation endproducts (RAGE). The pleiotropic action of vitamin D, especially calcitriol, may indicate a high therapeutic potential of vitamin D supplementation in various diseases with carbonyl stress etiology. Further research is needed to fully understand the underlying mechanisms of vitamin D pleiotropic effects and determine the optimal dosages for clinical use.