Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.4K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Counting the unseen: Quantifying interactions between proteins from single-molecule imaging in live cells.

Biophysical journal·2026
Same author

OptoLoop - an optogenetic tool to probe the functional role of genome organization.

Journal of cell science·2026
Same author

Quantification of SnRK1.1 response through analysis of its intracellular distribution.

Plant cell reports·2026
Same author

OptoLoop: An optogenetic tool to probe the functional role of genome organization.

bioRxiv : the preprint server for biology·2025
Same author

The multimerization pathway of the glucocorticoid receptor.

Nucleic acids research·2025
Same author

Hallmarks of glucocorticoid receptor condensates involvement in transcription regulation.

iScience·2025

Related Experiment Video

Updated: Nov 16, 2025

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

11.3K

Catching the glucocorticoid receptor in the act: Lessons from fluorescence fluctuation methods.

Martin Stortz1, Camila Oses2, Agustina L Lafuente3

  • 1Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), CONICET-Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina; Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.

Biochemical and Biophysical Research Communications
|January 17, 2025
PubMed
Summary

Fluorescence microscopy reveals transcription factors dynamically interact with chromatin, challenging previous deterministic models. Live-cell imaging of the glucocorticoid receptor advances understanding of gene regulation mechanisms.

Keywords:
FCSGene regulationGlucocorticoid receptorN&BSelf-associationTranscription

More Related Videos

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

11.4K
An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
10:00

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

14.5K

Related Experiment Videos

Last Updated: Nov 16, 2025

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

11.3K
High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

11.4K
An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
10:00

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

14.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biophysics

Background:

  • Eukaryotic mRNA synthesis was traditionally viewed as a sequential, deterministic process.
  • Advances in imaging reveal dynamic and stochastic interactions of transcriptional regulators with chromatin.

Purpose of the Study:

  • To review the contribution of fluorescence fluctuation methods to understanding transcription factor dynamics in live cells.
  • To focus on the glucocorticoid receptor as a model system for studying transcription factor mechanisms.

Main Methods:

  • Live-cell imaging techniques.
  • Fluorescence microscopy and fluctuation methods.
  • Analysis of transcription factor dynamics within the nucleus.

Main Results:

  • Demonstration of dynamic and probabilistic engagement of transcription factors with chromatin.
  • Elucidation of the mechanism of action for the glucocorticoid receptor.
  • Insights into context-specific gene network regulation by transcription factors.

Conclusions:

  • Fluorescence fluctuation methods have reshaped our understanding of eukaryotic transcription.
  • The glucocorticoid receptor serves as a valuable model for studying dynamic transcription factor behavior.
  • Live-cell imaging provides crucial data on the stochastic nature of gene regulation.