Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

49.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.3K
What is Genetic Engineering?00:49

What is Genetic Engineering?

73.5K
Overview
73.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.6K
Homologous Recombination02:31

Homologous Recombination

50.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Combination of Artemisinin, N-acetylcysteine, Resveratrol, and Hesperidin Ameliorates Hippocampal Damage and Pathological Features in an Experimental Model of Alzheimer's Disease.

Neurochemical research·2026
Same author

Geometry-dependent interfaces shape NLRP3 pyrin domain assembly.

International journal of biological macromolecules·2026
Same author

Applications of hollow-fiber systems in bone, liver, and pancreatic tissue engineering.

Tissue & cell·2026
Same author

Investigating synergistic effects of key medium components and pectin elicitor on taxane fermentation in Neopestalotiopsis vitis: A Box-Behnken response surface methodology approach.

International microbiology : the official journal of the Spanish Society for Microbiology·2026
Same author

Inhibitory effect of silymarin on amyloid formation in ALS-associated hSOD1 P66R mutant.

International journal of biological macromolecules·2026
Same author

Revolutionizing Stem Cell Sorting with Machine Learning: A Review of Trends, Tools, and Future Directions.

Iranian journal of medical sciences·2026

Related Experiment Video

Updated: Jun 1, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

508

CRISPR challenges in clinical developments.

Mohadeseh Khoshandam1, Hossein Soltaninejad2, Iman Bhia3

  • 1Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Qom, Iran; National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

Progress in Molecular Biology and Translational Science
|January 17, 2025
PubMed
Summary

CRISPR-Cas genome editing offers promising disease treatments but faces clinical implementation challenges. This review explores strategies to enhance CRISPR efficiency for genetic diseases and cancer, highlighting future prospects.

Keywords:
CRISPR-Cas9 systemChallengesClinical trialDelivery systemsOff-target effects

More Related Videos

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.1K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.5K

Related Experiment Videos

Last Updated: Jun 1, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

508
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.1K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.5K

Area of Science:

  • Biotechnology
  • Genetics
  • Molecular Biology

Background:

  • CRISPR-Cas is a revolutionary genome editing tool with significant therapeutic potential.
  • Current human applications are limited to clinical trials, despite FDA approvals and rapid growth.
  • Addressing challenges is vital for widespread clinical adoption of CRISPR technology.

Purpose of the Study:

  • To identify novel research directions for improving CRISPR efficiency in treating genetic diseases and cancer.
  • To explore the future prospects of CRISPR-Cas technology in clinical settings.
  • To provide a unique perspective on specific conditions, strategies, challenges, and opportunities in CRISPR research.

Main Methods:

  • Literature review focusing on CRISPR-Cas efficiency enhancement.
  • Analysis of specific conditions and strategies for CRISPR application.
  • Discussion of challenges and opportunities in the field.

Main Results:

  • Identification of research ideas to boost CRISPR efficiency for genetic disorders and cancer.
  • Exploration of CRISPR's future clinical applications and prospects.
  • Discussion of specific strategies and conditions relevant to CRISPR implementation.

Conclusions:

  • CRISPR-Cas technology holds immense promise for treating genetic diseases and cancer.
  • Overcoming current challenges is key to unlocking CRISPR's full clinical potential.
  • Continued research into efficiency, delivery, and specific applications will drive future advancements.