Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reproducible and sensitive detection of simple and complex glycan isomers using a new porous graphitized carbon phase packed in micro-bore geometries.

Talanta·2026

Same author

FUT8-Dependent Core Fucosylation: Essential for Platelet Function and a Target in Thrombosis.

Arteriosclerosis, thrombosis, and vascular biology·2026

Same author

TS3 Asn347 corticosteroid-binding globulin (CBG) glycoform deficiency is associated with mortality in human septic shock.

Journal of the Endocrine Society·2026

Same author

Mass spectrometry analysis of young and aged mice and human Alzheimer's disease with Lewy body pathology using on-slide tissue digestion.

Analytical and bioanalytical chemistry·2026

Same author

A novel glycoform of the rotavirus outer capsid protein VP7 is secreted from polarized cells and activates innate immune cells.

Journal of virology·2026

Same author

<i>PyCycleBio</i>: modelling non-sinusoidal-oscillator systems in temporal biology.

Bioinformatics advances·2026

Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026

Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026

Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026

Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026

Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026

Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 1, 2025

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Compositional data analysis enables statistical rigor in comparative glycomics.

Alexander R Bennett¹, Jon Lundstrøm^2,3, Sayantani Chatterjee⁴

¹Department of Medical Biochemistry, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.

Nature Communications

|January 17, 2025

Summary

This summary is machine-generated.

This study introduces a new compositional data analysis framework for comparative glycomics. It provides a robust pipeline to accurately analyze glycan data, controlling false-positive rates and revealing biological insights crucial for health and disease research.

More Related Videos

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Published on: January 20, 2022

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Related Experiment Videos

Last Updated: Jun 1, 2025

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Published on: January 20, 2022

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Area of Science:

Biochemistry
Bioinformatics
Systems Biology

Background:

Comparative glycomics data are compositional, with relative abundances of glycans.
Traditional statistical methods yield misleading conclusions due to data dependencies.

Purpose of the Study:

To develop a compositional data analysis framework for comparative glycomics.
To establish a statistically robust and sensitive data analysis pipeline for glycan data.

Main Methods:

Application of center log-ratio and additive log-ratio transformations.
Integration of a scale uncertainty/information model.
Development of alpha- and beta-diversity analyses and cross-class glycan correlations.

Main Results:

The framework controls false-positive rates in glycan analysis.
Reproducible biological findings are achieved with the new pipeline.
Specialized analyses reveal glycan interdependencies and variations.

Conclusions:

The proposed framework offers a statistically sound approach for comparative glycomics.
This method enhances the understanding of glycome variations in health and disease.
It enables deeper insights into the functional roles of glycans.