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Related Experiment Video

Updated: Jun 1, 2025

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Published on: June 24, 2012

Norovirus replication, host interactions and vaccine advances.

B V Venkataram Prasad^1,2, Robert L Atmar^2,3, Sasirekha Ramani²

¹Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, TX, USA.

Nature Reviews. Microbiology

|January 17, 2025

Summary

Human noroviruses (HuNoVs) cause widespread gastroenteritis, with no current vaccines or antivirals. Recent advances in cultivation and structural biology offer new insights into HuNoV strains and host interactions for future therapies.

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Last Updated: Jun 1, 2025

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

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Published on: June 24, 2012

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Published on: August 22, 2012

Area of Science:

Virology
Gastroenterology
Immunology

Background:

Human noroviruses (HuNoVs) are a primary cause of acute gastroenteritis globally.
Significant disease and economic burdens are associated with HuNoV infections.
Currently, no approved vaccines or antiviral therapies exist for HuNoV illness.

Purpose of the Study:

To review recent breakthroughs in understanding HuNoV biology.
To discuss advancements in virus cultivation, structural biology, and metagenomic sequencing.
To highlight strain-specific differences and virus-host interactions.

Main Methods:

Cultivation of HuNoVs using human intestinal organoid cultures.
Application of structural biology techniques and epitope mapping.
Utilizing metagenomic sequencing for strain analysis.

Main Results:

Breakthroughs in culturing HuNoVs have facilitated deeper biological understanding.
Structural biology and epitope mapping reveal unexpected strain-specific differences.
New insights into virus-host interactions and host factors influencing pathogenesis have emerged.

Conclusions:

Recent scientific advances are crucial for overcoming barriers in HuNoV vaccine and therapy development.
Understanding strain-specific variations in structure, replication, and host interactions is key.
Future research directions are informed by new knowledge of HuNoV pathogenesis.