Related Experiment Video

Updated: Jun 1, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.4K

Acetylation-enhanced Sp1 transcriptional activity suppresses Mlph expression

Chan Song Jo1, Hairu Zhao1, Jae Sung Hwang2

  • 1Department of Genetics and Biotechnology, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Korea.

Scientific Reports
|January 17, 2025
PubMed

Insights

Histone deacetylase 5 (HDAC5) regulates melanosome transport by controlling Melanophilin (Mlph) expression. This study reveals HDAC5

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Melanosome transport relies on the tripartite complex of Rab27a, Melanophilin (Mlph), and Myosin Va (Myo-Va).
  • Mutations in these proteins lead to melanosome aggregation near the nucleus.
  • While Mlph transcriptional regulation is studied, its post-translational modification control is not well understood.

Purpose of the Study:

  • To investigate the role of post-translational modifications in regulating Mlph expression.
  • To elucidate the specific mechanism by which HDAC5 influences melanosome transport.

Main Methods:

  • Inhibition of histone deacetylases (HDACs) using SAHA and TSA.
  • Knockdown of HDAC5.
  • Analysis of Sp1 acetylation and its binding to the Mlph promoter.

Main Results:

  • HDAC inhibition disrupted melanosome transport, causing aggregation.
  • HDAC5 was identified as a regulator of Mlph expression via Sp1.
  • HDAC5 knockdown increased Sp1 acetylation and its binding to the Mlph promoter, altering Mlph expression.

Conclusions:

  • HDAC5 plays a critical role in melanosome transport through its interaction with Sp1.
  • HDAC5-mediated deacetylation is a key post-translational modification in melanosome transport regulation.
  • This study provides novel insights into the molecular mechanisms governing melanosome transport.
Keywords:
AcetylationHDAC5MelanophilinMelanosome transportPost-translational modificationsSp1

More Related Videos

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

7.4K
Assays for Validating Histone Acetyltransferase Inhibitors09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K

Related Concept Videos

Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.1K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.2K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K