Protocol for reconstructing ancestral genomes from present-day samples by applying local ancestry inference
Related Experiment Video
Updated: Jun 1, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
Published on: November 30, 2021
Xiaoxi Zhang1, Baonan Wang2, Jia Wen2
1Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China; State Key Laboratory of Genetic Engineering, Human Phenome Institute, Zhangjiang Fudan International Innovation Center, Center for Evolutionary Biology, Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Abstract:
The genome of the most recent common ancestor is generally not available but can greatly facilitate the inference of demographic history and the detection of local adaptations. Here, we present a protocol for applying local ancestry inference in present-day samples to reconstruct ancestral genomes. We describe steps for estimating haplotypes, inferring local ancestry, and assembling ancestral haplotypes. This protocol describes the analytic steps of reconstructing ancestral genomes using the example data of the Miao and She target populations. For complete details on the use and execution of this protocol, please refer to Gao et al.1.
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Gene Evolution - Fast or Slow?
In contrast, regions which code...
Genome Annotation and Assembly
Synteny and Evolution
Around 80 million years ago, the human and mice lineages diverged from the common ancestor. During the course of evolution, the ancestral...
DNA Isolation

