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Rational design of hypochlorous acid-activatable fluorescent probe for diagnostic imaging and therapeutic evaluation in breast cancer recurrence

  • 0Department of Pharmacy, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology 430016 Wuhan, PR China.
Spectrochimica Acta. Part a, Molecular and Biomolecular Spectroscopy +

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January 18, 2025

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January 18, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A new fluorescent probe, QPIO, effectively detects hypochlorous acid (HClO) to monitor recurrent breast cancer (BC). This advancement aids in early diagnosis and tracking therapeutic progress for BC recurrence.

Area Of Science

  • Biomedical Engineering
  • Analytical Chemistry
  • Oncology

Background

  • Recurrent breast cancer (BC) poses a significant clinical challenge with rising mortality rates.
  • Current detection methods for BC recurrence are insufficient.
  • Hypochlorous acid (HClO) imbalance is linked to BC progression and metastasis, necessitating accurate monitoring.

Purpose Of The Study

  • To develop a novel fluorescent probe for sensitive and selective detection of hypochlorous acid (HClO).
  • To investigate the role of HClO in breast cancer (BC) recurrence.
  • To establish a tool for diagnosing and monitoring BC recurrence in vitro and in vivo.

Main Methods

  • Synthesis of a rational designed hypochlorous acid-activatable fluorescent probe (QPIO) based on phenothiazine, quinoline malononitrile, and hemicyanine.
  • In vitro detection of HClO levels in RAW 264.7 and 4T1 cells using QPIO.
  • In vivo fluorescence imaging in mouse models to correlate HClO levels with BC recurrence and therapeutic response.

Main Results

  • The synthesized QPIO probe exhibited high anti-interference capability and a significant Stokes shift for HClO detection.
  • QPIO successfully monitored intracellular HClO levels in various cell lines.
  • A direct correlation between dynamic HClO level changes and breast cancer recurrence was established in vivo.

Conclusions

  • QPIO is a promising tool for the early diagnosis and monitoring of recurrent breast cancer (BC).
  • The probe facilitates tracking therapeutic progress and understanding the biological role of HClO in BC recurrence.
  • This work provides a foundation for developing novel diagnostic and therapeutic strategies for recurrent BC.

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