Controlling As, Cd, and Pb bioaccumulation in rice under different levels of alternate wetting and drying irrigation...

One challenging task to produce rice that comply with the increasing demanding regulations, is to reduce, simultaneously, grain bioaccumulation of As, Cd, and Pb. A 3-year field experiment was conducted in a Mediterranean environment, to evaluate the effects on As, Cd, and Pb bioaccumulation in rice grain, of the adoption of two levels of alternate wetting and drying (AWD) irrigation conditions: moderate and intensive (reflooding at -20 kPa and -70 kPa soil matric water potential, respectively), relative to the traditional permanent flood irrigation. Plots were prepared with or without a one-time holm oak biochar application (35 Mg ha^-1), in the first year of the study. Arsenic bioaccumulation decreased in rice grain in the AWD systems, both total and inorganic (As_Inorg), with the lower values reached in the intensive AWD irrigation (0.131-0.151 mg kg^-1 dry weight), when the drying conditions were more intense. For As, biochar contributed to a further reduction in the bioaccumulation in the first two years but lost its efficacy with the field aging after three years of its application. However, the transition to AWD irrigation led to a significant increase in Cd bioaccumulation in rice grain (21-fold increase in the more intensive system, whose values reached up to 0.127 mg kg^-1), which can be counteracted by biochar application, to values statistically similar to those of permanent flooding. Contrariwise, the effects on Pb bioaccumulation were not so significant, but decreased with the transition to ADW irrigation, and with biochar application, relatively to the non-amended counterparts. Therefore, the implementation of intensive AWD with biochar represents a potentially fruitful strategy to enhance food safety of rice production, controlling, simultaneously, As, Cd, and Pb bioaccumulation. Nevertheless, new approaches need to be developed to attend the limits established for As_Inorg to produce food for infants, even in uncontaminated soils.