Blending polysaccharides from three edible mushrooms represents a promising approach for augmenting their...

It has been well documented that a number of polysaccharides with potent free-radical scavenging capability possess notable anti-fatigue activity. Interestingly, recent evidence also suggested mixed polysaccharides derived from multiple sources may yield augmented bioactivities compared to the polysaccharides from a single source. Therefore, in the current study, we investigated the anti-oxidant and anti-fatigue activities of a blend of polysaccharides isolated from three mushrooms. The optimal ratio of Pleurotus eryngii polysaccharide, Agrocybe aegerita polysaccharide, and Agaricus bisporus polysaccharide was firstly screened out by in vitro antioxidant assays with a Z-score comprehensive evaluation method. Then, the fatigue-reducing effects of the polysaccharide mixture at optimal ratio (1:2:2) were studied in mice. The results showed that the polysaccharide mixture exerted desirable fatigue-reducing effect evidence by significantly extended exhaustion time in the weight-loaded swimming assay (from 9.45 ± 3.06 min to 27.65 ± 19.84 min). Moreover, the observed fatigue-reducing effect may be attributed to its capacity to decrease fatigue-related metabolites, increase muscle glycogen reserves, and regulate energy metabolism-related transcripts in muscle tissue. Taken together, these findings highlighted the potential application of blending polysaccharides from multiple mushroom sources in mitigating fatigue.